| Item type |
文献 / Documents(1) |
| 公開日 |
2022-11-29 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1128/AAC.47.7.2093-2099.2003 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1128/AAC.47.7.2093-2099.2003 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Correlation between Resistance of Pseudomonas aeruginosa to Quaternary Ammonium Compounds and Expression of Outer Membrane Protein OprR |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
QAC RESISTANCE OF P. AERUGINOSA |
|
言語 |
en |
| 著者 |
田端, 厚之
長宗, 秀明
前田, 拓也
村上, 圭史
三宅, 洋一郎
(髙麗)高麗, 寛紀
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The adaptation mechanism of Pseudomonas aeruginosa ATCC 10145 to quaternary ammonium compounds (QACs) was investigated. A P. aeruginosa strain with adapted resistance to QACs was developed by a standard broth dilution method. It was revealed that P. aeruginosa exhibited remarkable resistance to N-dodecylpyridinium iodide (P-12), whose structure is similar to that of a common disinfectant, cetylpyridinium chloride. Adapted resistance to benzalkonium chloride (BAC), which is commonly used as a disinfectant, was also observed in P. aeruginosa. Moreover, the P-12-resistant strain exhibited cross-resistance to BAC. Analysis of the outer membrane protein of the P-12-resistant strain by two-dimensional polyacrylamide gel electrophoresis showed a significant increase in the level of expression of a protein (named OprR) whose molecular mass was approximately 26 kDa. The actual function of OprR is not yet clear; however, OprR was expected to be an outer membrane-associated protein with homology to lipoproteins of other bacterial species, according to a search of the National Center for Biotechnology Information website with the BLAST program by use of the N-terminal sequence of OprR. A correlation between the level of expression of OprR and the level of resistance of P. aeruginosa to QACs was observed by using a PA2800 gene knockout mutant derived from the P-12-resistant strain. The knockout mutant recovered susceptibility not only to P-12 but also to BAC. These results suggested that OprR significantly participated in the adaptation of P. aeruginosa to QACs, such as P-12 and BAC. |
|
言語 |
en |
| 書誌情報 |
en : Antimicrobial Agents and Chemotherapy
巻 47,
号 7,
p. 2093-2099,
発行日 2003-07-01
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
00664804 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
10986596 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00542574 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00542563 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12003089 |
| 出版者 |
|
|
出版者 |
American Society for Microbiology |
|
言語 |
en |
| EID |
|
|
識別子 |
73556 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
aac_47_7_2093.pdf (261 KB)
