| Item type |
文献 / Documents(1) |
| 公開日 |
2022-12-12 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1002/1873-3468.14520 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1002/1873-3468.14520 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Nonspecific phospholipase C3 of radish has phospholipase D activity towards glycosylinositol phosphoceramide |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Nonspecific phospholipase C3 of radish has phospholipase D activity toward glycosylinositol phosphoceramide |
|
言語 |
en |
| 著者 |
Hasi, Rumana Yesmin
イシカワ, トシキ
スナガワ, ケイゴ
タカイ, ヨシミチ
Ali, Hanif
林, 順司
川上, 竜巳
湯浅, 恵造
(中橋)粟飯原, 睦美
金丸, 芳
イマイ, ヒロユキ
田中, 保
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Previously, we found an enzyme activity that produces phytoceramide 1-phosphate (PC1P) by hydrolysis of D position of glycosylinositol phosphoceramide (GIPC) in cabbage and called its activity as GIPC-phospholipase D (PLD). Here, we purified GIPC-PLD by sequential chromatography from radish roots. Peptide mass fingerprinting analysis revealed that the potential candidate for GIPC-PLD protein was nonspecific phospholipase C3 (NPC3), which has not been characterized as PLD. The recombinant NPC3 protein obtained by heterologous expression system with E. coli produced PC1P from GIPC, and showed essentially the same enzymatic properties as those we characterized as GIPC-PLD in cabbage, radish, and A. thaliana. From these results, we concluded that NPC3 is one of the enzymes that degrade GIPC, a major sphingolipid in plants. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Glycosylinositol phosphoceramide |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
phospholipase D |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
phytoceramide 1-phosphate |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
nonspecific phospholipase C3 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Raphanus sativus |
| 書誌情報 |
en : FEBS Letters
巻 596,
号 23,
p. 3024-3036,
発行日 2022-10-20
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
18733468 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00642943 |
| 出版者 |
|
|
出版者 |
Federation of European Biochemical Societies |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Wiley |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is the peer reviewed version of the following article: Hasi, R.Y., Ishikawa, T., Sunagawa, K., Takai, Y., Ali, H., Hayashi, J., Kawakami, R., Yuasa, K., Aihara, M., Kanemaru, K., Imai, H. and Tanaka, T. (2022), Nonspecific phospholipase C3 of radish has phospholipase D activity towards glycosylinositol phosphoceramide. FEBS Lett. 596: 3024-3036. which has been published in final form at https://doi.org/10.1002/1873-3468.14520. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| EID |
|
|
識別子 |
392404 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
febslet_596_23_3024.pdf (1.76 MB)
