| Item type |
文献 / Documents(1) |
| 公開日 |
2022-12-12 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.3390/jdb10040046 |
|
|
関連名称 |
10.3390/jdb10040046 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Appropriate Amounts and Activity of the Wilms’ Tumor Suppressor Gene, wt1, Are Required for Normal Pronephros Development of Xenopus Embryos |
| 著者 |
シラキ, タイセイ
ハヤシ, タクマ
オズエ, ジョウタロウ
渡部, 稔
|
| 抄録 |
|
|
内容記述 |
The Wilms’ tumor suppressor gene, wt1, encodes a zinc finger-containing transcription factor that binds to a GC-rich motif and regulates the transcription of target genes. wt1 was first identified as a tumor suppressor gene in Wilms’ tumor, a pediatric kidney tumor, and has been implicated in normal kidney development. The WT1 protein has transcriptional activation and repression domains and acts as a transcriptional activator or repressor, depending on the target gene and context. In Xenopus, an ortholog of wt1 has been isolated and shown to be expressed in the developing embryonic pronephros. To investigate the role of wt1 in pronephros development in Xenopus embryos, we mutated wt1 by CRISPR/Cas9 and found that the expression of pronephros marker genes was reduced. In reporter assays in which known WT1 binding sequences were placed upstream of the luciferase gene, WT1 activated transcription of the luciferase gene. The injection of wild-type or artificially altered transcriptional activity of wt1 mRNA disrupted the expression of pronephros marker genes in the embryos. These results suggest that the appropriate amounts and activity of WT1 protein are required for normal pronephros development in Xenopus embryos. |
| キーワード |
|
|
主題 |
CRISPR/Cas9 |
| キーワード |
|
|
主題 |
pronephros |
| キーワード |
|
|
主題 |
transcription factor |
| キーワード |
|
|
主題 |
Wilms tumor |
| キーワード |
|
|
主題 |
WT1 |
| キーワード |
|
|
主題 |
Xenopus |
| 書誌情報 |
en : Journal of Developmental Biology
巻 10,
号 4,
p. 46,
発行日 2022-10-29
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
22213759 |
| 出版者 |
|
|
出版者 |
MDPI |
| 権利情報 |
|
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
393179 |
| 言語 |
|
|
言語 |
eng |
jdb_10_4_46.pdf (4.4 MB)
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