| Item type |
文献 / Documents(1) |
| 公開日 |
2023-03-09 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1248/bpb.b22-00873 |
|
|
関連名称 |
10.1248/bpb.b22-00873 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
The Effect of Iontophoretic-Delivered Polyplex Vaccine on Melanoma Regression |
| 著者 |
Husseini, Rabab A.
福田, 達也
大園, 瑞音
Hasan, Azza A.
El Megrab, Nagia A.
小暮, 健太朗
|
| 抄録 |
|
|
内容記述 |
Although the strategy in cancer vaccination is to provide a therapeutic effect against an established tumor, there is an urgent need to develop prophylactic vaccines for non-viral cancers. In this study, we prepared polyplex nanoparticles through electrostatic interactions between a positively-charged modified tumor associated antigen, namely human derived melanoma gp10025–33 peptide (KVPRNQDWL-RRRR), and a negatively charged cytosine-phosphate-guanosine motif (CpG-ODN) adjuvant. We previously demonstrated successful transdermal delivery of various hydrophilic macromolecules by iontophoresis (IP) using weak electricity. Herein, we investigated the effectiveness of IP in the transdermal delivery of a prophylactic polyplex vaccine. IP was successful in establishing a homogenous distribution of the vaccine throughout skin. Efficacy of the vaccine was demonstrated against melanoma growth. A significant tumor regression effect was observed, which was confirmed by elevated mRNA expression levels of various cytokines, mainly interferon (IFN)-γ, as well as infiltration of cytotoxic CD8+ T cells. Additionally, we evaluated the therapeutic effect of the vaccine and we found a significant reduction in tumor burden. Stimulation of systemic immunity was confirmed by upregulation of IFN-γ. This is the first report to demonstrate the use of IP in the transdermal delivery of a prophylactic melanoma vaccine. |
| キーワード |
|
|
主題 |
prophylactic cancer vaccine |
| キーワード |
|
|
主題 |
iontophoresis |
| キーワード |
|
|
主題 |
polyplex nanoparticle |
| キーワード |
|
|
主題 |
antigen peptide |
| キーワード |
|
|
主題 |
cytosine-phosphate- guanosine motif (CpG-ODN) adjuvant |
| キーワード |
|
|
主題 |
melanoma |
| 書誌情報 |
en : Biological and Pharmaceutical Bulletin
巻 46,
号 3,
p. 494-504,
発行日 2023-03-01
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13475215 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11696048 |
| 出版者 |
|
|
出版者 |
The Pharmaceutical Society of Japan |
| EID |
|
|
識別子 |
393581 |
| 言語 |
|
|
言語 |
eng |
bpb_46_3_494.pdf (1.88 MB)
