| Item type |
文献 / Documents(1) |
| 公開日 |
2023-03-10 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1091/mbc.E20-01-0031 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1091/mbc.E20-01-0031 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Conformational diversity of dynactin sidearm and domain organization of its subunit p150 |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Conformational diversity of dynactin |
|
言語 |
en |
| 著者 |
サイトウ, ケイ
ムラヤマ, タカシ
ハタ, トモネ
コバヤシ, タクヤ
柴田, 桂太朗
カズノ, サイコ
フジムラ, ツトム
サクライ, タカシ
トヨシマ, ヨウコ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Dynactin is a principal regulator of the minus-end directed microtubule motor dynein. The sidearm of dynactin is essential for binding to microtubules and regulation of dynein activity. Although our understanding of the structure of the dynactin backbone (Arp1 rod) has greatly improved recently, structural details of the sidearm subcomplex remain elusive. Here, we report the flexible nature and diverse conformations of dynactin sidearm observed by electron microscopy. Using nanogold labeling and deletion mutant analysis, we determined the domain organization of the largest subunit p150 and discovered that its coiled-coil (CC1), dynein-binding domain, adopted either a folded or an extended form. Furthermore, the entire sidearm exhibited several characteristic forms, and the equilibrium among them depended on salt concentrations. These conformational diversities of the dynactin complex provide clues to understanding how it binds to microtubules and regulates dynein. |
|
言語 |
en |
| 書誌情報 |
en : Molecular Biology of the Cell
巻 31,
号 12,
p. 1218-1231,
発行日 2020-05-28
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
19394586 |
| 出版者 |
|
|
出版者 |
The American Society for Cell Biology |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). |
| EID |
|
|
識別子 |
380110 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
mboc_31_12_1218.pdf (2.65 MB)
.png)
