| Item type |
文献 / Documents(1) |
| 公開日 |
2023-08-22 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1248/bpb.b23-00135 |
|
|
関連名称 |
10.1248/bpb.b23-00135 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Development of Functional Chimeric Nanoparticles by Membrane Fusion of Small Extracellular Vesicles and Drug-Encapsulated Liposomes |
| 著者 |
福田, 達也
ニシカワ, アキナ
ヒラマチ, アミ
ヤマシタ, サチカ
小暮, 健太朗
|
| 抄録 |
|
|
内容記述 |
Since small extracellular vesicle (sEVs) are involved in cell-to-cell communication via transfer of certain bioactive molecules and have the capability to overcome biological barriers against drug transport, their use as a drug delivery system (DDS) has been demonstrated in treatment of a diverse range of diseases. However, some issues in drug encapsulation have been pointed out, including low encapsulation efficiency and poor reproducibility. It was previously reported that liposomes containing phosphatidylserine (PS) can fuse together in the presence of calcium ion, which allows for drug encapsulation into the resultant liposomes (i.e., calcium fusion method). On the other hand, PS is reportedly present in lipid membrane of sEVs as a distinct lipid composition. We therefore hypothesized that PS-mediated membrane fusion of sEVs with PS-liposomes encapsulating therapeutic agents via the calcium fusion method can be applied to convenient drug encapsulation into sEVs. Membrane fusion of PS-liposomes and sEVs derived from murine melanoma B16F1 cells (B16-sEVs) was firstly confirmed. The obtained nanoparticles, termed chimeric nanoparticles (CM-NP), showed comparable cellular uptake to B16-sEVs into B16F1 cells. Moreover, CM-NP encapsulating an anticancer drug doxorubicin (DOX) (CM-NP-DOX) could be prepared by membrane fusion of PS-liposomes encapsulating DOX (PS-Lipo-DOX) and B16-sEVs. CM-NP-DOX exhibited a superior anticancer effect on B16F1 cells in vitro compared with PS-Lipo-DOX. These findings suggest that the calcium fusion method could be applied for membrane fusion of sEVs and PS-liposomes, and that this approach would likely be useful for efficient drug encapsulation into sEVs, as well as increasing liposome functionality. |
| キーワード |
|
|
主題 |
small extracellular vesicle |
| キーワード |
|
|
主題 |
liposome |
| キーワード |
|
|
主題 |
drug delivery system |
| キーワード |
|
|
主題 |
phosphatidylserine |
| キーワード |
|
|
主題 |
calcium fusion |
| 書誌情報 |
en : Biological and Pharmaceutical Bulletin
巻 46,
号 8,
p. 1098-1104,
発行日 2023-08-01
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13475215 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11696048 |
| 出版者 |
|
|
出版者 |
The Pharmaceutical Society of Japan |
| EID |
|
|
識別子 |
397825 |
| 言語 |
|
|
言語 |
eng |
bpb_46_8_1098.pdf (1.42 MB)
