| Item type |
文献 / Documents(1) |
| 公開日 |
2023-08-31 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1016/j.jmoldx.2022.03.010 |
|
|
関連名称 |
10.1016/j.jmoldx.2022.03.010 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Epigenome-Wide DNA Methylation Profiling of Normal Mucosa Reveals HLA-F Hypermethylation as a Biomarker Candidate for Serrated Polyposis Syndrome |
| タイトル別表記 |
|
|
その他のタイトル |
DNA Methylation Profiling of SPS |
| 著者 |
Jung, Gerhard
Hernández-Illán, Eva
Lozano, Juan J.
Sidorova, Julia
Muñoz, Jenifer
岡田, 泰行
Quintero, Enrique
Hernandez, Goretti
Jover, Rodrigo
Carballal, Sabela
Cuatrecasas, Miriam
Moreno, Lorena
Diaz, Mireia
Ocaña, Teresa
Sánchez, Ariadna
Rivero, Liseth
Ortiz, Oswaldo
Llach, Joan
Castells, Antoni
Pellisé, Maria
Goel, Ajay
Batlle, Eduard
Balaguer, Francesc
|
| 抄録 |
|
|
内容記述 |
Serrated polyposis syndrome (SPS) is associated with a high risk for colorectal cancer. Intense promoter hypermethylation is a frequent molecular finding in the serrated pathway and may be present in normal mucosa, predisposing to the formation of serrated lesions. To identify novel biomarkers for SPS, fresh-frozen samples of normal mucosa from 50 patients with SPS and 19 healthy individuals were analyzed by using the 850K BeadChip Technology (Infinium). Aberrant methylation levels were correlated with gene expression using a next-generation transcriptome profiling tool. Two validation steps were performed on independent cohorts: first, on formalin-fixed, paraffin-embedded tissue of the normal mucosa; and second, on 24 serrated lesions. The most frequently hypermethylated genes were HLA-F, SLFN12, HLA-DMA, and RARRES3; and the most frequently hypomethylated genes were PIWIL1 and ANK3 (Δβ = 10%; P < 0.05). Expression levels of HLA-F, SLFN12, and HLA-DMA were significantly different between SPS patients and healthy individuals and correlated well with the methylation status of the corresponding differentially methylated region (fold change, >20%; r > 0.55; P < 0.001). Significant hypermethylation of CpGs in the gene body of HLA-F was also found in serrated lesions (Δβ = 23%; false discovery rate = 0.01). Epigenome-wide methylation profiling has revealed numerous differentially methylated CpGs in normal mucosa from SPS patients. Significant hypermethylation of HLA-F is a novel biomarker candidate for SPS. |
| 書誌情報 |
en : The Journal of Molecular Diagnostics
巻 24,
号 6,
p. 674-686,
発行日 2022-04-18
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15251578 |
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
19437811 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11395041 |
| 出版者 |
|
|
出版者 |
Association for Molecular Pathology |
| 出版者 |
|
|
出版者 |
American Society for Investigative Pathology |
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利情報 |
|
|
権利情報 |
Open Archive |
| EID |
|
|
識別子 |
399621 |
| 言語 |
|
|
言語 |
eng |
jmold_24_6_674.pdf (1.79 MB)
