| Item type |
文献 / Documents(1) |
| 公開日 |
2023-11-28 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1507/endocrj.EJ18-0441 |
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関連名称 |
10.1507/endocrj.EJ18-0441 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Development of monoclonal mouse antibodies that specifically recognize pancreatic polypeptide |
| タイトル別表記 |
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その他のタイトル |
PP-specific monoclonal antibodies |
| 著者 |
ハラ, アケミ
ナカガワ, ユウコ
ナカオ, ケイコ
田蒔, 基行
池本, 哲也
島田, 光生
松久, 宗英
ミズカミ, ヒロキ
マルヤマ, ノブヒロ
ワタダ, ヒロタカ
フジタニ, ヨシオ
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| 抄録 |
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内容記述 |
Pancreatic polypeptide (PP) is a 36-amino acid peptide encoded by the Ppy gene, which is produced by a small population of cells located in the periphery of the islets of Langerhans. Owing to the high amino acid sequence similarity among neuropeptide Y family members, antibodies against PP that are currently available are not convincingly specific to PP. Here we report the development of mouse monoclonal antibodies that specifically bind to PP. We generated Ppy knockout (Ppy-KO) mice in which the Ppy-coding region was replaced by Cre recombinase. The Ppy-KO mice were immunized with mouse PP peptide, and stable hybridoma cell lines producing anti-PP antibodies were isolated. Firstly, positive clones were selected in an enzyme-linked immunosorbent assay for reactivity with PP coupled to bovine serum albumin. During the screening, hybridoma clones producing antibodies that cross-react to the peptide YY (PYY) were excluded. In the second screening, hybridoma clones in which their culture media produce no signal in Ppy-KO islets but detect specific cells in the peripheral region of wild-type islets, were selected. Further studies demonstrated that the selected monoclonal antibody (23-2D3) specifically recognizes PP-producing cells, not only in mouse, but also in human and rat islets. The monoclonal antibodies with high binding specificity for PP developed in this study will be fundamental for future studies towards elucidating the expression profiles and the physiological roles of PP. |
| キーワード |
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|
主題 |
Islet |
| キーワード |
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|
主題 |
Pancreatic polypeptide (PP) |
| キーワード |
|
|
主題 |
Peptide YY |
| キーワード |
|
|
主題 |
Neuropeptide Y |
| キーワード |
|
|
主題 |
Monoclonal antibody |
| 書誌情報 |
en : Endocrine Journal
巻 66,
号 5,
p. 459-468,
発行日 2019
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13484540 |
| 出版者 |
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|
出版者 |
The Japan Endocrine Society |
| EID |
|
|
識別子 |
352296 |
| 言語 |
|
|
言語 |
eng |
endocrj_66_5_459.pdf (5.83 MB)
