| Item type |
文献 / Documents(1) |
| 公開日 |
2024-02-16 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1021/acs.jpcb.3c00302 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1021/acs.jpcb.3c00302 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Simultaneous Imaging and Characterization of Polyunsaturated Fatty Acids, Carotenoids, and Microcrystalline Guanine in Single Aurantiochytrium limacinum Cells with Linear and Nonlinear Raman Microspectroscopy |
|
言語 |
en |
| 著者 |
ササキ, リサ
トダ, ショウゴ
阪本, 鷹行
櫻谷, 英治
シゲトウ, シンスケ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Thraustochytrids are heterotrophic marine protists known for their high production capacity of various compounds with health benefits, such as polyunsaturated fatty acids and carotenoids. Although much effort has been focused on developing optimal cultivation methods for efficient microbial production, these high-value compounds and their interrelationships are not well understood at the single-cell level. Here we used spontaneous (linear) Raman and multiplex coherent anti-Stokes Raman scattering (CARS) micro-spectroscopy to visualize and characterize lipids (e.g., docosahexaenoic acid) and carotenoids (e.g., astaxanthin) accumulated in single living Aurantiochytrium limacinum cells. Spontaneous Raman imaging with the help of multivariate curve resolution−alternating least-squares enabled us to make unambiguous assignments of the molecular components we detected and derive their intracellular distributions separately. Near-IR excited CARS imaging yielded the Raman images at least an order of magnitude faster than spontaneous Raman imaging, with suppressed contributions of carotenoids. As the culture time increased from 2 to 5 days, the lipid amount increased by a factor of ∼7, whereas the carotenoid amount did not change significantly. Furthermore, we observed a highly localized component in A. limacinum cells. This component was found to be mixed crystals of guanine and other purine derivatives. The present study demonstrates the potential of the linear−nonlinear Raman hybrid approach that allows for accurate molecular identification and fast imaging in a label-free manner to link information derived from single cells with strategies for mass culture of useful thraustochytrids. |
|
言語 |
en |
| 書誌情報 |
en : The Journal of Physical Chemistry B
巻 127,
号 12,
p. 2708-2718,
発行日 2023-03-15
|
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15206106 |
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15205207 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11114073 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12189704 |
| 出版者 |
|
|
出版者 |
ACS Publications |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an open access article under the CC BY-NC-ND license. |
| EID |
|
|
識別子 |
394716 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jpcb_127_12_2708.pdf (5.42 MB)
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