| Item type |
文献 / Documents(1) |
| 公開日 |
2024-01-24 |
| アクセス権 |
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アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.1093/jb/mvad080 |
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|
関連名称 |
10.1093/jb/mvad080 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Degradation of glycosylinositol phosphoceramide during plant tissue homogenization |
| タイトル別表記 |
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その他のタイトル |
GIPC Degradation in Plants |
| 著者 |
タカイ, ヨシミチ
Hasi, Rumana Yesmin
マツモト, ナオコ
フジタ, チホ
Ali, Hanif
林, 順司
川上, 竜巳
(中橋)粟飯原, 睦美
イシカワ, トシキ
イマイ, ヒロユキ
ワキダ, マユコ
アンドウ, カズヤ
田中, 保
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| 抄録 |
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|
内容記述 |
A convenient method for the determination of plant sphingolipids (glycosylinositol phosphoceramide, GIPC; glucosylceramide, GluCer; phytoceramide 1-phosphate, PC1P and phytoceramide, PCer) was developed. This method includes the extraction of lipids using 1-butanol, alkali hydrolysis with methylamine and separation by TLC. The amounts of sphingolipids in the sample were determined based on the relative intensities of standard sphingolipids visualized by primulin/ UV on TLC. Using this method, we found that almost all GIPC were degraded in response to tissue homogenization in cruciferous plants (cabbage, broccoli and Arabidopsis thaliana). The decrease in GIPC was compensated for by increases in PC1P and PCer, indicating that GIPC was degraded by hydrolysis at the D and C positions of GIPC, respectively. In carrot roots and leaves, most of GIPC degradation was compensated for by an increase in PCer. In rice roots, the decrease in GIPC was not fully explained by the increases in PC1P and PCer, indicating that enzymes other than phospholipase C and D activities operated. As the visualization of lipids on TLC is useful for detecting the appearance or disappearance of lipids, this method will be available for the characterization of metabolism of sphingolipids in plants. |
| キーワード |
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|
主題 |
glycosylinositol phosphoceramide |
| キーワード |
|
|
主題 |
phospholipase C |
| キーワード |
|
|
主題 |
phospholipase D |
| キーワード |
|
|
主題 |
phytoceramide |
| キーワード |
|
|
主題 |
phytoceramide 1-phosphate |
| 書誌情報 |
en : The Journal of Biochemistry
巻 175,
号 1,
p. 115-124,
発行日 2023-10-12
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
17562651 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0021924X |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12096002 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00694073 |
| 出版者 |
|
|
出版者 |
Oxford University Press |
| 権利情報 |
|
|
権利情報 |
This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Biochemistry following peer review. The version of record Yoshimichi Takai, Rumana Yesmin Hasi, Naoko Matsumoto, Chiho Fujita, Hanif Ali, Junji Hayashi, Ryushi Kawakami, Mutsumi Aihara, Toshiki Ishikawa, Hiroyuki Imai, Mayuko Wakida, Kazuya Ando, Tamotsu Tanaka, Degradation of glycosylinositol phosphoceramide during plant tissue homogenization, The Journal of Biochemistry, Volume 175, Issue 1, January 2024, Pages 115–124, is available online at: https://doi.org/10.1093/jb/mvad080. |
| EID |
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識別子 |
403391 |
| 言語 |
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言語 |
eng |
jb_175_1_115.pdf (2.93 MB)
