| Item type |
文献 / Documents(1) |
| 公開日 |
2024-07-05 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1093/ndt/gfaa156 |
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関連名称 |
10.1093/ndt/gfaa156 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Evidence of an intestinal phosphate transporter alternative to type IIb sodium-dependent phosphate transporter in rats with chronic kidney disease |
| タイトル別表記 |
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その他のタイトル |
Alternative intestinal phosphate transporter in CKD |
| 著者 |
イチダ, ヤスヒロ
オオトモ, シュウイチ
ヤマモト, テッサイ
ムラオ, ナオアキ
ツボイ, ヨシノリ
カワベ, ヨシキ
瀬川, 博子
ホリバ, ナオシ
宮本, 賢一
Floege, Jürgen
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| 抄録 |
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内容記述 |
Background: Phosphate is absorbed in the small intestine via passive flow and active transport.NaPi-IIb, a type II sodium-dependent phosphate transporter, is considered to mediate active phosphate transport in rodents. To study the regulation of intestinal phosphate transport in chronic kidney disease (CKD), we analyzed the expression levels of NaPi-IIb, pituitary-specific transcription factor 1 (PiT-1) and PiT-2 and the kinetics of intestinal phosphate transport using two CKD models.
Methods: CKD was induced in rats via adenine orThy1 anti-body injection. Phosphate uptake by intestinal brush border membrane vesicles (BBMV) and the messenger RNA (mRNA) expression of NaPi-IIb, PiT-1 and PiT-2 were analyzed. The protein expression level of NaPi-IIb was measured by mass spectrometry (e.g. liquid chromatography tandem mass spectrometry).
Results: In normal rats, phosphate uptake into BBMV consisted of a single saturable component and its Michaelis constant (Km) was comparable to that of NaPi-IIb. The maximum velocity (Vmax) correlated with mRNA and protein levels of NaPi-IIb. In the CKD models, intestinal phosphate uptake consisted of two saturable components. The Vmax of the higher-affinity transport, which is thought to be responsible for NaPi-IIb, significantly decreased and the decrease correlated with reduced NaPi-IIb expression. The Km of the lower-affinity transport was comparable to that of PiT-1 and -2. PiT-1 mRNA expression was much higher than that of PiT-2, suggesting that PiT-1 was mostly responsible for phosphate transport.
Conclusions: This study suggests that the contribution of NaPi-IIb to intestinal phosphate absorption dramatically decreases in rats with CKD and that a low-affinity alternative to NaPi-IIb, in particular PiT-1, is upregulated in a compensatory manner in CKD. |
| キーワード |
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|
主題 |
chronic kidney disease |
| キーワード |
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主題 |
intestine |
| キーワード |
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|
主題 |
NaPi-IIb |
| キーワード |
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主題 |
phosphate PiT |
| 書誌情報 |
en : Nephrology Dialysis Transplantation
巻 36,
号 1,
p. 68-75,
発行日 2020-09-03
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
09310509 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
14602385 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA1073277X |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12096159 |
| 出版者 |
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出版者 |
Oxford University Press |
| 権利情報 |
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|
権利情報 |
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
| EID |
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識別子 |
379563 |
| 言語 |
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言語 |
eng |
ndt_36_1_68.pdf (616 KB)
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