| Item type |
文献 / Documents(1) |
| 公開日 |
2024-07-05 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1093/nar/gkad387 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1093/nar/gkad387 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Epigenetic plasticity safeguards heterochromatin configuration in mammals |
|
言語 |
en |
| 著者 |
フクダ, ケイ
シミ, タケシ
シムラ, チカコ
オノ, タカオ
スズキ, タケヒロ
オノウエ, ケンタ
オカヤマ, サトコ
ミウラ, ヒサシ
ヒラタニ, イチロウ
イケダ, カズホ
オカダ, ヤスシ
ドウマエ, ナオシ
米村, 重信
イノウエ, アズサ
キムラ, ヒロシ
シンカイ, ヨウイチ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Heterochromatin is a key architectural feature of eukaryotic chromosomes critical for cell type-specific gene expression and genome stability. In the mammalian nucleus, heterochromatin segregates from transcriptionally active genomic regions and exists in large, condensed, and inactive nuclear compartments. However, the mechanisms underlying the spatial organization of heterochromatin need to be better understood. Histone H3 lysine 9 trimethylation (H3K9me3) and lysine 27 trimethylation (H3K27me3) are two major epigenetic modifications that enrich constitutive and facultative heterochromatin, respectively. Mammals have at least five H3K9 methyltransferases (SUV39H1, SUV39H2, SETDB1, G9a and GLP) and two H3K27 methyltransferases (EZH1 and EZH2). In this study, we addressed the role of H3K9 and H3K27 methylation in heterochromatin organization using a combination of mutant cells for five H3K9 methyltransferases and an EZH1/2 dual inhibitor, DS3201. We showed that H3K27me3, which is normally segregated from H3K9me3, was redistributed to regions targeted by H3K9me3 after the loss of H3K9 methylation and that the loss of both H3K9 and H3K27 methylation resulted in impaired condensation and spatial organization of heterochromatin. Our data demonstrate that the H3K27me3 pathway safeguards heterochromatin organization after the loss of H3K9 methylation in mammalian cells. |
|
言語 |
en |
| 書誌情報 |
en : Nucleic Acids Research
巻 51,
号 12,
p. 6190-6207,
発行日 2023-05-13
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13624962 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
03051048 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00760269 |
| 出版者 |
|
|
出版者 |
Oxford University Press |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
| EID |
|
|
識別子 |
399960 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
nar_51_12_6190.pdf (2.97 MB)
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