| Item type |
文献 / Documents(1) |
| 公開日 |
2024-03-05 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.ijbiomac.2022.03.114 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1016/j.ijbiomac.2022.03.114 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Crystal structure of a novel type of ornithine δ-aminotransferase from the hyperthermophilic archaeon Pyrococcus horikoshii |
|
言語 |
en |
| 著者 |
川上, 竜巳
オオシダ, タツヤ
林, 順司
ヨネダ, カズナリ
フルモト, トシオ
大島, 敏久
櫻庭, 春彦
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Ornithine δ-aminotransferase (Orn-AT) activity was detected for the enzyme annotated as a γ-aminobutyrate aminotransferase encoded by PH1423 gene from Pyrococcus horikoshii OT-3. Crystal structures of this novel archaeal ω-aminotransferase were determined for the enzyme in complex with pyridoxal 5′-phosphate (PLP), in complex with PLP and l-ornithine (l-Orn), and in complex with N-(5′-phosphopyridoxyl)-l-glutamate (PLP-l-Glu). Although the sequence identity was relatively low (28%), the main-chain coordinates of P. horikoshii Orn-AT monomer showed notable similarity to those of human Orn-AT. However, the residues recognizing the α-amino group of l-Orn differ between the two enzymes. In human Orn-AT, Tyr55 and Tyr85 recognize the α-amino group, whereas the side chains of Thr92* and Asp93*, which arise from a loop in the neighboring subunit, form hydrogen bonds with the α-amino group of the substrate in P. horikoshii enzyme. Site-directed mutagenesis suggested that Asp93* plays critical roles in maintaining high affinity for the substrate. This study provides new insight into the substrate binding of a novel type of Orn-AT. Moreover, the structure of the enzyme with the reaction-intermediate analogue PLP-l-Glu bound provides the first structural evidence for the “Glu switch” mechanism in the dual substrate specificity of Orn-AT. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ornithine aminotransferase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Archaea |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Crystal structure |
| 書誌情報 |
en : International Journal of Biological Macromolecules
巻 208,
p. 731-740,
発行日 2022-03-23
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
01418130 |
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
18790003 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00233999 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA1153092X |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
|
|
識別子 |
385357 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
ijbiomac_208_731.pdf (4.65 MB)
