| Item type |
文献 / Documents(1) |
| 公開日 |
2024-04-23 |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1002/jrs.6665 |
|
|
関連名称 |
10.1002/jrs.6665 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Raman microspectroscopy for label-free diagnosis of amyloid light-chain amyloidosis in various organs |
| 著者 |
柳谷, 伸一郎
ホンダ, タケシ
髙成, 広起
曽我部, 公子
中村, 信元
坂東, 良美
常山, 幸一
安倍, 正博
三木, 浩和
|
| 抄録 |
|
|
内容記述 |
Systemic amyloidosis is a group of diseases in which misfolded proteins aggregate as fibrous amyloid proteins with a β-sheet structure and deposit in organs, resulting in organ failure. Most types of amyloidosis have a poor prognosis, and prompt diagnosis is essential for treatment. Amyloid light-chain (AL) amyloidosis is a type of systemic amyloidosis that occurs when abnormal immunoglobulin light chain proteins are deposited in various organs and tissues. The deposition of amyloid proteins in tissues has traditionally been confirmed using Congo red staining and polarised light microscopy, which show apple-green birefringence. In this study, we aimed to verify whether amyloid deposition in the heart, kidney, rectum, duodenum, and skin can be detected using Raman microspectroscopy. Serial sections were prepared from formalin-fixed paraffin-embedded tissue biopsy samples obtained from patients with systemic amyloidosis. One of the serial sections was stained with Congo red to confirm the deposition of amyloid proteins using polarised light microscopy, whereas the other was left unstained for Raman microspectroscopy. A characteristic peak at Raman shift of 1665-1680 cm-1, which may represent a β-sheet structure of amyloid proteins, was recorded in the area where the amyloid deposition had been confirmed by Congo red staining. Based on the peak at 1640–1680 cm-1, a colour map was obtained to detect amyloid protein-positive regions. Thus, amyloid protein detection using Raman microspectroscopy may be useful for rapid diagnosis of amyloidosis. |
| キーワード |
|
|
主題 |
amyloidosis |
| キーワード |
|
|
主題 |
conformation change of proteins |
| キーワード |
|
|
主題 |
light microscopy |
| キーワード |
|
|
主題 |
Raman imaging |
| 書誌情報 |
en : Journal of Raman Spectroscopy
巻 55,
号 7,
p. 753-760,
発行日 2024-03-18
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
10974555 |
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03770486 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00705861 |
| 出版者 |
|
|
出版者 |
John Wiley & Sons |
| 権利情報 |
|
|
権利情報 |
This is the peer reviewed version of the following article: Shin-ichiro Yanagiya, Takeshi Honda, Hiroki Takanari, Kimiko Sogabe, Shingen Nakamura, Yoshimi Bando, Koichi Tsuneyama, Masahiro Abe, Hirokazu Miki, Raman microspectroscopy for label-free diagnosis of amyloid light-chain amyloidosis in various organs. Journal of Raman Spectroscopy. 55, 7, 753-760, which has been published in final form at https://doi.org/10.1002/jrs.6665. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| EID |
|
|
識別子 |
405345 |
| 言語 |
|
|
言語 |
eng |
jrs_55_7_753.pdf (916 KB)
