| Item type |
文献 / Documents(1) |
| 公開日 |
2024-04-19 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
関連識別子 |
https://doi.org/10.1007/s10266-023-00789-x |
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|
関連名称 |
10.1007/s10266-023-00789-x |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
β-defensin 2 synthesized by a cell-free protein synthesis system and encapsulated in liposomes inhibits adhesion of Porphyromonas gingivalis to oral epithelial cells |
| 著者 |
廣島, 佑香
木戸, 淳一
木戸, 理恵
吉田, 賀弥
板東, 美香
カジモト, カズアキ
湯本, 浩通
篠原, 康雄
|
| 抄録 |
|
|
内容記述 |
β-defensin 2 (BD-2), an antimicrobial peptide (AMP), is expressed by oral epithelial cells and plays an important role in innate immunity of the oral cavity. Cell-free protein synthesis (CFPS) systems have been studied for the synthesis of various proteins, however, the synthesis of BD-2 by a CFPS system has not been extensively explored. Liposomes have been developed as tools for drug delivery. A delivery of liposome-encapsulated AMP to oral epithelium may be useful to prevent oral infectious diseases. In the present study, we investigated the antimicrobial activity of the BD-2 protein, artificially synthesized using a CFPS system and encapsulated in liposomes. BD-2 protein was artificially synthesized using template DNA and a reconstituted CFPS system and was identified by western blotting. Bilayer liposomes were prepared using 1,2-dioleoyl-sn-glycero-3-phospho-choline and 3-sn-phosphatidylcholine from egg yolk. The artificially synthesized BD-2 was encapsulated in liposomes, collected by ultrafiltration, and detected by western blotting. Human oral epithelial cells were cultured with the liposome-encapsulated BD-2 and the concentration of BD-2 in the cell lysate of the culture with the synthesized BD-2 was higher than that of the control cultures. The antimicrobial activity of the synthesized BD-2 was investigated by an adhesion assay of Porphyromonas gingivalis to oral epithelial cells. The artificially synthesized BD-2 and its liposome significantly inhibited adhesion of P. gingivalis to oral epithelial cells. These results suggest that artificially synthesized BD-2 and liposome-encapsulated BD-2 shows antimicrobial activity and can potentially play a role in oral healthcare for periodontal diseases. |
| キーワード |
|
|
主題 |
β-defensin 2 |
| キーワード |
|
|
主題 |
Cell-free protein synthesis system |
| キーワード |
|
|
主題 |
Liposome |
| キーワード |
|
|
主題 |
Drug delivery system |
| キーワード |
|
|
主題 |
Porphyromonas gingivalis |
| 書誌情報 |
en : Odontology
巻 111,
号 4,
p. 830-838,
発行日 2023-02-06
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
16181255 |
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
16181247 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11580928 |
| 出版者 |
|
|
出版者 |
Springer Nature |
| 出版者 |
|
|
出版者 |
The Society of The Nippon Dental University |
| 備考 |
|
|
値 |
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use (https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms), but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s10266-023-00789-x |
| EID |
|
|
識別子 |
394013 |
| 言語 |
|
|
言語 |
eng |
odontology_111_4_830.pdf (664 KB)
