Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells

木戸, 淳一; キド, ジュンイチ; Kido, Jun-ichi; 廣島, 佑香; ヒロシマ, ユカ; Hiroshima, Yuka; 木戸, 理恵; キド, リエ; Kido, Rie; 吉田, 賀弥; ヨシダ, カヤ; Yoshida, Kaya; 稲垣, 裕司; イナガキ, ユウジ; Inagaki, Yuji; 成石, 浩司; ナルイシ, コウジ; Naruishi, Koji; カジモト, カズアキ; カジモト, カズアキ; Kajimoto, Kazuaki; 片岡, 正俊; カタオカ, マサトシ; Kataoka, Masatoshi; 篠原, 康雄; シノハラ, ヤスオ; Shinohara, Yasuo; 湯本, 浩通; ユモト, ヒロミチ; Yumoto, Hiromichi

doi:https://doi.org/10.1111/jre.13088

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Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells

https://tokushima-u.repo.nii.ac.jp/records/2011911

名前 / ファイル	ライセンス	アクション
jpr_58_2_262.pdf (1.14 MB)

Item type

文献 / Documents(1)

公開日

2024-04-19

アクセス権

open access

資源タイプ

資源タイプ識別子

http://purl.org/coar/resource_type/c_6501

資源タイプ

journal article

出版社版DOI

識別子タイプ

DOI

関連名称

10.1111/jre.13088

出版タイプ

出版タイプResource

http://purl.org/coar/version/c_ab4af688f83e57aa

タイトル

Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells

言語

著者

木戸, 淳一

WEKO 1669
e-Rad 10195315

ja	木戸, 淳一 ISNI
ja-Kana	キド, ジュンイチ
en	Kido, Jun-ichi

Search repository

廣島, 佑香

WEKO 414
徳島大学教育研究者総覧 204938/profile-ja.html
e-Rad 60545143

ja	廣島, 佑香 ISNI
ja-Kana	ヒロシマ, ユカ
en	Hiroshima, Yuka

Search repository

木戸, 理恵

WEKO 1006
徳島大学教育研究者総覧 364591/profile-ja.html
e-Rad 60876027

ja	木戸, 理恵 ISNI
ja-Kana	キド, リエ
en	Kido, Rie

Search repository

吉田, 賀弥

WEKO 1211
徳島大学教育研究者総覧 73175/profile-ja.html
e-Rad 60363157

ja	吉田, 賀弥 ISNI
ja-Kana	ヨシダ, カヤ
en	Yoshida, Kaya

Search repository

稲垣, 裕司

WEKO 1261
徳島大学教育研究者総覧 83114/profile-ja.html
e-Rad 50380019

ja	稲垣, 裕司 ISNI
ja-Kana	イナガキ, ユウジ
en	Inagaki, Yuji

Search repository

成石, 浩司

WEKO 713
e-Rad 00346446

ja	成石, 浩司 ISNI
ja-Kana	ナルイシ, コウジ
en	Naruishi, Koji

Search repository

カジモト, カズアキ

ja	カジモト, カズアキ
ja-Kana	カジモト, カズアキ
en	Kajimoto, Kazuaki

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片岡, 正俊

ja	片岡, 正俊
ja-Kana	カタオカ, マサトシ
en	Kataoka, Masatoshi

Search repository

篠原, 康雄

WEKO 1126
徳島大学教育研究者総覧 60617/profile-ja.html
e-Rad 60226157

ja	篠原, 康雄 ISNI
ja-Kana	シノハラ, ヤスオ
en	Shinohara, Yasuo

Search repository

湯本, 浩通

WEKO 1691
徳島大学教育研究者総覧 60390/profile-ja.html
e-Rad 60284303

ja	湯本, 浩通 ISNI
ja-Kana	ユモト, ヒロミチ
en	Yumoto, Hiromichi

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抄録

内容記述タイプ

Abstract

内容記述

Background and objective: Lipocalin 2 (LCN2), a glycoprotein expressed in epithelial cells and leukocytes, has an antibacterial effect and plays a role in innate immunity. The delivery of LCN2 encapsulated in liposomes to oral epithelium may be useful to prevent oral infectious diseases. This study aimed to investigate the inhibitory effect of LCN2, artificially synthesized using a cell-free protein synthesis (CFPS) system, on the adhesion of Porphyromonas gingivalis to oral epithelial cells in order to approach oral healthcare using LCN2.
Methods: LCN 2 was synthesized using a CFPS system and assayed by Western blotting, mass spectrometry and enzyme-linked immunosorbent assay (ELISA). The bilayer liposomes were prepared by the spontaneous transfer method using 1,2-dioleoyl-sn-glycero-3 phosphocholine (DOPC), 3-sn-phosphatidylcholine from Egg Yolk (Egg-PC), and 1,2-dioleoyl-sn-glycero-3 phosphoethanolamine (DOPE). The cellular and medium fractions derived from the culture of oral epithelial cells with liposome-encapsulated LCN2 were assayed by Western blotting and ELISA. The effect of the synthesized LCN2 on adhesion of the labeled P. gingivalis to oral epithelial cells was investigated as an evaluation of its antibacterial activity.
Results: The synthesized LCN2 protein was identified by Western blotting; its amino acid sequence was similar to that of recombinant LCN2 protein. The additions of DOPE and octa-arginine in the outer lipid-layer components of liposome significantly increased the delivery of liposomes to epithelial cells.When oral epithelial cells were cultured with the synthesized and liposome-encapsulated LCN2, LCN2 was identified in the cellular and medium fractions by Western blotting and its concentration in the cellular fraction from the culture with the synthesized LCN2 was significantly higher than that of a template DNA-free protein. The synthesized LCN2 and liposome-encapsulated LCN2 significantly inhibited the adhesion of P. gingivalis to oral epithelial cells compared with template DNA-free protein.
Conclusion: LCN2 was artificially synthesized by a CFPS system, encapsulated in liposomes and delivered to oral epithelial cells, and demonstrated an antibacterial action against P. gingivalis. This approach may become a useful model for oral healthcare.

言語

キーワード

言語

主題Scheme

Other

主題

antimicrobial peptide

キーワード

言語

主題Scheme

Other

主題

cell-free protein synthesis

キーワード

言語

主題Scheme

Other

主題

drug delivery system

キーワード

言語

主題Scheme

Other

主題

Lipocalin 2

キーワード

言語

主題Scheme

Other

主題

liposome

キーワード

言語

主題Scheme

Other

主題

oral epithelial cells

キーワード

言語

主題Scheme

Other

主題

oral healthcare

書誌情報

en : Journal of Periodontal Research

巻 58, 号 2, p. 262-273, 発行日 2022-12-29

収録物ID

収録物識別子タイプ

ISSN

収録物識別子

00223484

収録物ID

収録物識別子タイプ

ISSN

収録物識別子

16000765

収録物ID

収録物識別子タイプ

NCID

収録物識別子

AA00704381

収録物ID

収録物識別子タイプ

NCID

収録物識別子

AA11628616

出版者

Wiley

言語

権利情報

言語

権利情報

This is the peer reviewed version of the following article: Jun-ichi Kido, Yuka Hiroshima, Rie Kido, Kaya Yoshida, Yuji Inagaki, Koji Naruishi, Kazuaki Kajimoto, Masatoshi Kataoka, Yasuo Shinohara, Hiromichi Yumoto, Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells. Journal of Periodontal Research. 2023, 58, 2, 262-273., which has been published in final form at https://doi.org/10.1111/jre.13088. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

EID

識別子

394011

識別子タイプ

URI

言語

eng

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Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells

× 木戸, 淳一

× 廣島, 佑香

× 木戸, 理恵

× 吉田, 賀弥

× 稲垣, 裕司

× 成石, 浩司

× カジモト, カズアキ

× 片岡, 正俊

× 篠原, 康雄

× 湯本, 浩通

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