WEKO3
アイテム
Advanced glycation end-products and Porphyromonas gingivalis lipopolysaccharide increase calprotectin expression in human gingival epithelial cells
https://tokushima-u.repo.nii.ac.jp/records/2011912
https://tokushima-u.repo.nii.ac.jp/records/20119124b5ec9e8-f45d-4a32-88e2-793ab00a1865
| 名前 / ファイル | ライセンス | アクション |
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jcb_119_2_1591.pdf (3.55 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||||
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| 公開日 | 2024-04-19 | |||||||||||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1002/jcb.26319 | |||||||||||||||||||||||||||||||
| 言語 | ja | |||||||||||||||||||||||||||||||
| 関連名称 | 10.1002/jcb.26319 | |||||||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||||||
| タイトル | Advanced glycation end-products and Porphyromonas gingivalis lipopolysaccharide increase calprotectin expression in human gingival epithelial cells | |||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||
| タイトル別表記 | ||||||||||||||||||||||||||||||||
| その他のタイトル | EFFECTS OF AGE AND PGLPS ON CALPROTECTIN | |||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||
| 著者 |
廣島, 佑香
× 廣島, 佑香
WEKO
414
× 坂本, 英次郎
× 吉田, 賀弥
WEKO
1211
× 阿部, 佳織× 成石, 浩司× 山本, 武範× 篠原, 康雄
WEKO
1126
× 木戸, 淳一× Geczy, Carolyn L.
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| 抄録 | ||||||||||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||||||||||
| 内容記述 | Accumulation of advanced glycation end-products (AGEs) in periodontal tissues of patients with diabetes mellitus aggravates periodontitis, but the mechanisms are unknown. Calprotectin, a heterocomplex of S100A8 and S100A9 proteins, is a constitutive cytoplasmic component of healthy gingival epithelial cells. This study aimed at investigating the effects of AGE and Porphyromonas gingivalis lipopolysaccharide (PgLPS) on calprotectin expression in the human gingival epithelial cell line OBA-9. AGE and PgLPS increased the expression of S100A8 and S100A9 mRNAs, and AGE+PgLPS co-stimulation amplified their expression in OBA-9 cells. A higher concentration of calprotectin in cell lysates was also induced by stimulation with AGE and/or PgLPS. S100A8 was mainly translocated from the nucleus to the cytoplasm by AGE stimulation, while cytoplasmic localization of S100A9 was not altered following stimulation with AGE and/or PgLPS. Calprotectin was found in the cytoplasm of BSA-treated cells, but cytoplasmic and nuclear localization was observed following stimulation with AGE and/or PgLPS. AGE-induced S100A8, and S100A9 mRNA expression was partially suppressed by RAGE-specific siRNA. In contrast, PgLPS-induced S100A8 and S100A9 mRNA expression was strongly suppressed by TLR2-specific siRNA. Furthermore, the inhibition of p38, JNK MAPK, and NF-κB attenuated AGE- and PgLPS-induced S100A8 and S100A9 mRNA expression. Taken together, these results demonstrate that AGE acts in synergy with PgLPS to stimulate RAGE and TLR2 expression and activate p38, JNK MAPK, and NF-κB signaling pathways, resulting in increased activation of calprotectin (S100A8/S100A9) in human gingival epithelial cells. Our results suggest that calprotectin may be involved in the pathogenesis of diabetic periodontitis. | |||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||||||||||
| 主題 | advanced glycation end-products | |||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||||||||||
| 主題 | calprotectin | |||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||||||||||
| 主題 | human gingival epithelial cells | |||||||||||||||||||||||||||||||
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| 言語 | en | |||||||||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||||||||||
| 主題 | Porphyromonas gingivalis lipopolysaccharide | |||||||||||||||||||||||||||||||
| 書誌情報 |
en : Journal of Cellular Biochemistry 巻 119, 号 2, p. 1591-1603, 発行日 2017-08-03 |
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| 収録物識別子 | 07302312 | |||||||||||||||||||||||||||||||
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| 収録物識別子 | 10974644 | |||||||||||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||||||||||
| 収録物識別子 | AA1052210X | |||||||||||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||||||||||
| 収録物識別子 | AA11622653 | |||||||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||||||
| 出版者 | Wiley Periodicals | |||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||||
| 権利情報 | This is the peer reviewed version of the following article: Yuka Hiroshima, Eijiro Sakamoto, Kaya Yoshida, Kaori Abe, Koji Naruishi, Takenori Yamamoto, Yasuo Shinohara, Jun-ichi Kido, Carolyn L. Geczy, Advanced glycation end-products and Porphyromonas gingivalis lipopolysaccharide increase calprotectin expression in human gingival epithelial cells. Journal of Cellular Biochemistry. 2018, 119, 2, 1591-1603., which has been published in final form at https://doi.org/10.1002/jcb.26319. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. | |||||||||||||||||||||||||||||||
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| 識別子 | 327751 | |||||||||||||||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||||
