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Increased Lipogenesis Is Important for Hexavalent Chromium-Transformed Lung Cells and Xenograft Tumor Growth
https://tokushima-u.repo.nii.ac.jp/records/2011955
https://tokushima-u.repo.nii.ac.jp/records/20119556e522663-ddce-4cb4-a5ef-9aa42ddf9a7e
| 名前 / ファイル | ライセンス | アクション |
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ijms_24_23_17060.pdf (3.39 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||
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| 公開日 | 2024-06-14 | |||||||||||||
| アクセス権 | ||||||||||||||
| アクセス権 | open access | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 出版社版DOI | ||||||||||||||
| 関連識別子 | https://doi.org/10.3390/ijms242317060 | |||||||||||||
| 関連名称 | 10.3390/ijms242317060 | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Increased Lipogenesis Is Important for Hexavalent Chromium-Transformed Lung Cells and Xenograft Tumor Growth | |||||||||||||
| 著者 |
Wise, James T. F.
× Wise, James T. F.
× 近藤, 和也
WEKO
1540
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| 抄録 | ||||||||||||||
| 内容記述 | Hexavalent chromium, Cr(VI), is a known carcinogen and environmental health concern. It has been established that reactive oxygen species, genomic instability, and DNA damage repair deficiency are important contributors to the Cr(VI)-induced carcinogenesis mechanism. However, some hallmarks of cancer remain under-researched regarding the mechanism behind Cr(VI)-induced carcinogenesis. Increased lipogenesis is important to carcinogenesis and tumorigenesis in multiple types of cancers, yet the role increased lipogenesis has in Cr(VI) carcinogenesis is unclear. We report here that Cr(VI)-induced transformation of three human lung cell lines (BEAS-2B, BEP2D, and WTHBF-6) resulted in increased lipogenesis (palmitic acid levels), and Cr(VI)-transformed cells had an increased expression of key lipogenesis proteins (ATP citrate lyase [ACLY], acetyl-CoA carboxylase [ACC1], and fatty acid synthase [FASN]). We also determined that the Cr(VI)-transformed cells did not exhibit an increase in fatty acid oxidation or lipid droplets compared to their passage-matched control cells. Additionally, we observed increases in ACLY, ACC1, and FASN in lung tumor tissue compared with normal-adjacent lung tissue (in chromate workers that died of chromate-induced tumors). Next, using a known FASN inhibitor (C75), we treated Cr(VI)-transformed BEAS-2B with this inhibitor and measured cell growth, FASN protein expression, and growth in soft agar. We observed that FASN inhibition results in a decreased protein expression, decreased cell growth, and the inhibition of colony growth in soft agar. Next, using shRNA to knock down the FASN protein in Cr(VI)-transformed BEAS-2B cells, we saw a decrease in FASN protein expression and a loss of the xenograft tumor development of Cr(VI)-transformed BEAS-2B cells. These results demonstrate that FASN is important for Cr(VI)-transformed cell growth and cancer properties. In conclusion, these data show that Cr(VI)-transformation in vitro caused an increase in lipogenesis, and that this increase is vital for Cr(VI)-transformed cells. | |||||||||||||
| キーワード | ||||||||||||||
| 主題 | hexavalent chromium | |||||||||||||
| キーワード | ||||||||||||||
| 主題 | lipogenesis | |||||||||||||
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| 主題 | cancer metabolism | |||||||||||||
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| 主題 | fatty acid synthase | |||||||||||||
| キーワード | ||||||||||||||
| 主題 | human lung cells | |||||||||||||
| 書誌情報 |
en : International Journal of Molecular Sciences 巻 24, 号 23, p. 17060, 発行日 2023-12-02 |
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| 収録物ID | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 14220067 | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | MDPI | |||||||||||||
| 権利情報 | ||||||||||||||
| 権利情報 | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | |||||||||||||
| EID | ||||||||||||||
| 識別子 | 404648 | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
