| Item type |
文献 / Documents(1) |
| 公開日 |
2024-05-15 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1038/s41598-023-48887-8 |
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関連名称 |
10.1038/s41598-023-48887-8 |
| 出版タイプ |
|
|
出版タイプ |
NA |
|
出版タイプResource |
http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| タイトル |
|
|
タイトル |
Quantification of collagen fiber properties in alcoholic liver fibrosis using polarization‑resolved second harmonic generation microscopy |
| タイトル別表記 |
|
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その他のタイトル |
偏光分解顕微鏡を用いたアルコール性肝線維症の線維化の量的・質的評価系の開発 |
| 著者 |
松崎, 紗弥
長谷, 栄治
髙成, 広起
ハヤシ, ユリ
ハヤシ, ユウサク
オシカタ, ハルト
南川, 丈夫
キムラ, サトコ
(市村)清水, 真祐子
安井, 武史
原田, 雅史
常山, 幸一
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| 抄録 |
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内容記述 |
Liver fibrosis is assessed mainly by conventional staining or second harmonic generation (SHG) microscopy, which can only provide collagen content in fibrotic area. We propose to use polarization-resolved SHG (PR-SHG) microscopy to quantify liver fibrosis in terms of collagen fiber orientation and crystallization. Liver samples obtained from autopsy cases with fibrosis stage of F0–F4 were evaluated with an SHG microscope, and 12 consecutive PR-SHG images were acquired while changing the polarization azimuth angle of the irradiated laser from 0° to 165° in 15° increments using polarizer. The fiber orientation angle (φ) and degree (ρ) of collagen were estimated from the images. The SHG-positive area increased as the fibrosis stage progressed, which was well consistent with Sirius Red staining. The value of φ was random regardless of fibrosis stage. The mean value of ρ (ρ-mean), which represents collagen fiber crystallinity, varied more as fibrosis progressed to stage F3, and converged to a significantly higher value in F4 than in other stages. Spatial dispersion of ρ (ρ-entropy) also showed increased variation in the stage F3 and decreased variation in the stage F4. It was shown that PR-SHG could provide new information on the properties of collagen fibers in human liver fibrosis. |
| キーワード |
|
|
主題 |
SHGimage |
| キーワード |
|
|
主題 |
hepatic scleosis |
| キーワード |
|
|
主題 |
collagen fiber |
| キーワード |
|
|
主題 |
fatty liver disease |
| 書誌情報 |
en : Scientific Reports
巻 13,
p. 22100,
発行日 2023-12-13
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| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20452322 |
| 出版者 |
|
|
出版者 |
Springer Nature |
| 備考 |
|
|
値 |
内容要旨・審査要旨・論文本文の公開
本論文は，著者Saya Matsuzakiの学位論文として提出され，学位審査・授与の対象となっている。
学位授与者所属 : 徳島大学大学院医学研究科（医学専攻） |
| 権利情報 |
|
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
|
|
識別子 |
406337 |
| 言語 |
|
|
言語 |
eng |
| 報告番号 |
|
|
学位授与番号 |
甲第3785号 |
| 学位記番号 |
|
|
値 |
甲医第1600号 |
| 学位授与年月日 |
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|
学位授与年月日 |
2024-03-07 |
| 学位名 |
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学位名 |
博士（医学） |
| 学位授与機関 |
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学位授与機関名 |
徳島大学 |
k3785_abstract.pdf (147 KB)
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