| Item type |
文献 / Documents(1) |
| 公開日 |
2024-11-20 |
| アクセス権 |
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|
アクセス権 |
embargoed access |
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アクセス権URI |
http://purl.org/coar/access_right/c_f1cf |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.jchromb.2024.124318 |
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言語 |
ja |
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|
関連名称 |
10.1016/j.jchromb.2024.124318 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
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|
タイトル |
Microdialysis-integrated HPLC system with dual-electrode detection using track-etched membrane electrodes for in vivo monitoring of dopamine dynamics |
|
言語 |
en |
| 著者 |
オガワ, ユクト
ツギタ, ソウヘイ
トリイ, ユウカ
イワモト, ヒテン
サトウ, ツカサ
笠原, 二郎
竹内, 政樹
桑原, 知彦
イイヤマ, マサミツ
髙栁, 俊夫
水口, 仁志
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| 抄録 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
A capillary high-performance liquid chromatography (HPLC) system equipped with a dual-electrode detector utilizing track-etched membrane electrodes (TEMEs) was combined with a microdialysis sampling setup. The electrochemical detector benefits from the high electrolysis efficiency of TEMEs, allowing for calibration-free coulometric detection and simplifying data analysis to determine the dopamine recovery through a dialysis probe. Additionally, this system was used for in vivo monitoring of dopamine in the right striatum of a mouse brain. Temporal changes in dopamine levels, including an exponential decay immediately after the dialysis probe insertion and an excess release of dopamine induced by a high concentration of potassium ions, confirmed the system's proper operation. Furthermore, subsequent measurements following the intraperitoneal injection of mirtazapine showed no increase in dopamine levels in the right dorsal striatum. The dual-electrode system displayed characteristic dopamine detection behavior, with anodic and cathodic peak pairs indicative of reversible electrochemical reactions. This capability facilitated the identification of the dopamine peak within the complex chromatogram of the mouse brain dialysate. The consistency between dopamine collection efficiency from standard solutions and dialysate indicated the absence of interfering electroactive substances overlapping with the dopamine peak in the chromatogram. This integrated analysis system successfully tracked temporal fluctuations in dopamine concentration within the mouse brain. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Track-etched membrane electrodes (TEMEs) |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Dual-electrode detection |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Coulometric detection |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Dopamine |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
In vivo microdialysis |
| 書誌情報 |
en : Journal of Chromatography B
巻 1247,
p. 124318,
発行日 2024-09-14
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| 収録物ID |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
15700232 |
| 収録物ID |
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|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1873376X |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11644805 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11656178 |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
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|
識別子 |
414429 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jchromb_1247_124318.pdf (637 KB)
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