| Item type |
文献 / Documents(1) |
| 公開日 |
2024-11-20 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/ijms252011252 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3390/ijms252011252 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Rectal Epithelial Stem Cell Kinetics in Acute Radiation Proctitis |
|
言語 |
en |
| 著者 |
Ghosh, Sharmila
森田, 明典
西山, 祐一
サカウエ, マサヒロ
フジワラ, ケン
モリタ, ダイキ
ソノヤマ, ユウイチロウ
ヒガシ, ユウイチ
ササタニ, メグミ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The intestinal tract is a typical radiosensitive tissue, and radiation rectal injury is a severe side effect that limits the prescribed dose in radiotherapy of the abdominal and pelvic region. Understanding the post-irradiation kinetics of Lgr5-positive stem cells is crucial in comprehending this adverse process. In this study, we utilized Lgr5-EGFP knock-in mice expressing EGFP and LGR5 antibody fluorescence staining of wild-type mice. At the state of radiation injury, the qPCR analysis showed a significant decrease in the expression level of Lgr5 in the rectal epithelial tissue. The dose-response relationship analysis showed that at low to moderate doses up to 10 gray (Gy), Lgr5-clustered populations were observed at the base of the crypt, whereas at sublethal doses (20 Gy and 29 Gy), the cells exhibited a dot-like scatter pattern, termed Lgr5-dotted populations. During recovery, 30 days post-irradiation, Lgr5-clustered populations gradually re-emerged while Lgr5-dotted populations declined, implying that some of the Lgr5-dotted stem cell populations re-clustered, aiding regenerations. Based on statistical analysis of the dose-response relationship using wild-type mice, the threshold dose for destroying these stem cell structures is 18 Gy. These findings may help set doses in mouse abdominal irradiation experiments for radiation intestinal injury and for understanding the histological process of injury development. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
mouse model |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
radiation proctitis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Lgr5-positive stem cell |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
crypt base regeneration |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
caudal half-body irradiation |
| 書誌情報 |
en : International Journal of Molecular Sciences
巻 25,
号 20,
p. 11252,
発行日 2024-10-19
|
| 収録物ID |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
14220067 |
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
414990 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
ijms_25_20_11252.pdf (6.88MB)
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