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Phosphorylated ubiquitin chain is the genuine Parkin receptor
https://tokushima-u.repo.nii.ac.jp/records/2012718
https://tokushima-u.repo.nii.ac.jp/records/2012718b490e46d-f5c7-41bc-bdaf-d3d37db8649b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
jcb_209_1_111.pdf (4.6 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2025-04-04 | |||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1083/jcb.201410050 | |||||||||||||||||||||||
| 関連名称 | 10.1083/jcb.201410050 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Phosphorylated ubiquitin chain is the genuine Parkin receptor | |||||||||||||||||||||||
| 著者 |
Okatsu, Kei
× Okatsu, Kei
× Koyano, Fumika
× Kimura, Mayumi
× 小迫, 英尊
WEKO
301
× Saeki, Yasushi
× Tanaka, Keiji
× Matsuda, Noriyuki
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| 抄録 | ||||||||||||||||||||||||
| 内容記述 | PINK1 selectively recruits Parkin to depolarized mitochondria for quarantine and removal of damaged mitochondria via ubiquitylation. Dysfunction of this process predisposes development of familial recessive Parkinson’s disease. Although various models for the recruitment process have been proposed, none of them adequately explain the accumulated data, and thus the molecular basis for PINK1 recruitment of Parkin remains to be fully elucidated. In this study, we show that a linear ubiquitin chain of phosphomimetic tetra-ubiquitin(S65D) recruits Parkin to energized mitochondria in the absence of PINK1, whereas a wild-type tetra-ubiquitin chain does not. Under more physiologically relevant conditions, a lysosomal phosphorylated polyubiquitin chain recruited phosphomimetic Parkin to the lysosome. A cellular ubiquitin replacement system confirmed that ubiquitin phosphorylation is indeed essential for Parkin translocation. Furthermore, physical interactions between phosphomimetic Parkin and phosphorylated polyubiquitin chain were detected by immunoprecipitation from cells and in vitro reconstitution using recombinant proteins. We thus propose that the phosphorylated ubiquitin chain functions as the genuine Parkin receptor for recruitment to depolarized mitochondria. | |||||||||||||||||||||||
| 書誌情報 |
en : Journal of Cell Biology 巻 209, 号 1, p. 111-128, 発行日 2015-04-06 |
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||||||
| 収録物識別子 | 15408140 | |||||||||||||||||||||||
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| 収録物識別子タイプ | PISSN | |||||||||||||||||||||||
| 収録物識別子 | 00219525 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA00694812 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA1203766X | |||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | Rockefeller University Press | |||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||
| 権利情報 | This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). | |||||||||||||||||||||||
| EID | ||||||||||||||||||||||||
| 識別子 | 295274 | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
