| Item type |
文献 / Documents(1) |
| 公開日 |
2025-03-14 |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1016/j.jbc.2023.103040 |
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関連名称 |
10.1016/j.jbc.2023.103040 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
s-Afadin binds to MAGUIN/Cnksr2 and regulates the localization of the AMPA receptor and glutamatergic synaptic response in hippocampal neurons |
| タイトル別表記 |
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その他のタイトル |
s-Afadin−MAGUIN interaction regulates synaptic functions |
| 著者 |
Maruo, Tomohiko
水谷, 清人
宮田, 宗明
Kuriu, Toshihiko
榊原, 正太郎
WEKO
991
| ja |
榊原, 正太郎
ISNI
|
| ja-Kana |
サカキバラ, ショウタロウ
|
| en |
Sakakibara, Shotaro
|
Search repository
Takahashi, Hatena
Kida, Daichi
Maesaka, Kouki
Sugaya, Tsukiko
坂根, 亜由子
佐々木, 卓也
Takai, Yoshimi
Mandai, Kenji
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| 抄録 |
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内容記述 |
A hippocampal mossy fiber synapse implicated in learning and memory is a complex structure in which a presynaptic bouton attaches to the dendritic trunk by puncta adherentia junctions (PAJs) and wraps multiply branched spines. The postsynaptic densities (PSDs) are localized at the heads of each of these spines and faces to the presynaptic active zones. We previously showed that the scaffolding protein afadin regulates the formation of the PAJs, PSDs, and active zones in the mossy fiber synapse. Afadin has two splice variants: l-afadin and s-afadin. l-Afadin, but not s-afadin, regulates the formation of the PAJs but the roles of s-afadin in synaptogenesis remain unknown. We found here that s-afadin more preferentially bound to MAGUIN (a product of the Cnksr2 gene) than l-afadin in vivo and in vitro. MAGUIN/CNKSR2 is one of the causative genes for nonsyndromic X-linked intellectual disability accompanied by epilepsy and aphasia. Genetic ablation of MAGUIN impaired PSD-95 localization and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic (AMPA) receptor surface accumulation in cultured hippocampal neurons. Our electrophysiological analysis revealed that the postsynaptic response to glutamate, but not its release from the presynapse, was impaired in the MAGUIN-deficient cultured hippocampal neurons. Furthermore, disruption of MAGUIN did not increase the seizure susceptibility to flurothyl, a GABAA receptor antagonist. These results indicate that s-afadin binds to MAGUIN and regulates the PSD-95-dependent cell surface localization of the AMPA receptor and glutamatergic synaptic responses in the hippocampal neurons and that MAGUIN is not involved in the induction of epileptic seizure by flurothyl in our mouse model. |
| 書誌情報 |
en : Journal of Biological Chemistry
巻 299,
号 4,
p. 103040,
発行日 2023-02-18
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| 収録物ID |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1083351X |
| 収録物ID |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
00219258 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA1202441X |
| 出版者 |
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出版者 |
American Society for Biochemistry and Molecular Biology |
| 出版者 |
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出版者 |
Elsevier |
| 権利情報 |
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|
権利情報 |
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| EID |
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|
識別子 |
412518 |
| 言語 |
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|
言語 |
eng |
jbc_299_4_103040.pdf (1.7 MB)
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