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Conformational Distribution of a Multidomain Protein Measured by Single-Pair Small-Angle X-ray Scattering
https://tokushima-u.repo.nii.ac.jp/records/2012833
https://tokushima-u.repo.nii.ac.jp/records/201283379d5a1f2-bdf1-4d6e-9527-de522768b9e0
| 名前 / ファイル | ライセンス | アクション |
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jpcl_15_3_744.pdf (2 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||
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| 公開日 | 2025-03-21 | |||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1021/acs.jpclett.3c02600 | |||||||||||||||||||||
| 関連名称 | 10.1021/acs.jpclett.3c02600 | |||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | Conformational Distribution of a Multidomain Protein Measured by Single-Pair Small-Angle X-ray Scattering | |||||||||||||||||||||
| 著者 |
Kawamukai, Honoka
× Kawamukai, Honoka
× Takishita, Shumpei
× Shimizu, Kazumi
× Kohda, Daisuke
× Ishimori, Koichiro
× 齋尾, 智英
WEKO
1188
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| 抄録 | ||||||||||||||||||||||
| 内容記述 | The difficulty in evaluating the conformational distribution of proteins in solution often hinders mechanistic insights. One possible strategy for visualizing conformational distribution is distance distribution measurement by single-pair small-angle X-ray scattering (SAXS), in which the scattering interference from only a specific pair of atoms in the target molecule is extracted. Despite this promising concept, with few applications in synthetic small molecules and DNA, technical difficulties have prevented its application in protein conformational studies. This study used a synthetic tag to fix the lanthanide ion at desired sites on the protein and used single-pair SAXS with contrast matching to evaluate the conformational distribution of the multidomain protein enzyme MurD. These data highlighted the broad conformational and ligand-driven distribution shifts of MurD in solution. This study proposes an important strategy in solution structural biology that targets dynamic proteins, including multidomain and intrinsically disordered proteins. | |||||||||||||||||||||
| 書誌情報 |
en : Journal of Physical Chemistry Letters 巻 15, 号 3, p. 744-750, 発行日 2024-01-14 |
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||||
| 収録物識別子 | 19487185 | |||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||
| 収録物識別子 | AA1260018X | |||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||
| 出版者 | ACS Publications | |||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||
| 権利情報 | This article is licensed under CC-BY 4.0. | |||||||||||||||||||||
| EID | ||||||||||||||||||||||
| 識別子 | 412433 | |||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
