| Item type |
文献 / Documents(1) |
| 公開日 |
2024-10-21 |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| item_1722929371688 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.xgen.2024.100510 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1016/j.xgen.2024.100510 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
In vivo CRISPR screening directly targeting testicular cells |
|
言語 |
en |
| 著者 |
Noguchi, Yuki
Onodera, Yasuhito
Miyamoto, Tatsuo
Maruoka, Masahiro
小迫, 英尊
Suzuki, Jun
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
CRISPR-Cas9 short guide RNA (sgRNA) library screening is a powerful approach to understand the molecular mechanisms of biological phenomena. However, its in vivo application is currently limited. Here, we developed our previously established in vitro revival screening method into an in vivo one to identify factors involved in spermatogenesis integrity by utilizing sperm capacitation as an indicator. By introducing an sgRNA library into testicular cells, we successfully pinpointed the retinal degeneration 3 (Rd3) gene as a significant factor in spermatogenesis. Single-cell RNA sequencing (scRNA-seq) analysis highlighted the high expression of Rd3 in round spermatids, and proteomics analysis indicated that Rd3 interacts with mitochondria. To search for cell-type-specific signaling pathways based on scRNA-seq and proteomics analyses, we developed a computational tool, Hub-Explorer. Through this, we discovered that Rd3 modulates oxidative stress by regulating mitochondrial distribution upon ciliogenesis induction. Collectively, our screening system provides a valuable in vivo approach to decipher molecular mechanisms in biological processes. |
|
言語 |
en |
| bibliographic_information |
en : Cell Genomics
巻 4,
号 3,
p. 100510,
発行日 2024-03-05
|
| 収録物ID |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2666979X |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| item_10001_rights_15 |
|
|
言語 |
en |
|
権利情報 |
This is an open access article unde rthe CC BY-NC-ND license. |
| item_1723180141928 |
|
|
識別子 |
409523 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
cgen_4_3_100510.pdf (7.1 MB)
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