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Effects of ketamine on nicorandil induced ATP-sensitive potassium channel activity in cell line derived from rat aortic smooth muscle
https://tokushima-u.repo.nii.ac.jp/records/2001353
https://tokushima-u.repo.nii.ac.jp/records/2001353795aa6a8-50a1-4561-b5e4-8eb30f6489ad
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
LID201112132005.pdf (537 KB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||
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| 公開日 | 2011-12-13 | |||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Effects of ketamine on nicorandil induced ATP-sensitive potassium channel activity in cell line derived from rat aortic smooth muscle | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 著者 |
河野, 崇
× 河野, 崇
× 田中, 克哉× Yin, hua
× 江口, 覚× 河野, 裕明
× 髙橋, 章× 中屋, 豊× 大下, 修造 |
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| 抄録 | ||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||
| 内容記述 | Purpose : Nicorandil opens adenosine triphosphate-sensitive potassium (KATP) channels in the cardiovascular system and is being increasingly used for the treatment of angina pectoris. In the present study, we tested whether intravenous anesthetic agent ketamine affected nicorandil-induced native vascular KATP channel activation. Methods : We used excised inside-out patch clamp configurations to investigate the direct effects of ketamine racemate and S-(+)-ketamine on the activities of KATP channels in cultured rat aortic smooth muscle cells. Furthermore, we also investigated whether intracellular MgADP could modulate ketamine inhibition. Results : Nicorandil significantly activated KATP channel activity, whereas this channel activity was completely blocked by glibenclamide, a specific KATP channel blocker. Ketamine racemate inhibited the nicorandil induced KATP channel activity (IC50=34 1M, n=14), but S-(+)-ketamine was less potent than ketamine racemate in blocking nicorandil induced KATP channel activities (IC50=226 7M, n=10). Application of MgADP to the intracellular side of the channel was able to decrease the inhibitory potency of ketamine racemate on nicorandil induced KATP channel activities. Conclusions : Our results indicate that ketamine inhibits nicorandil induced KATP channel activities in a dose dependent and stereoselective manner. Furthermore, increase of intracellularMgADP attenuates the inhibitory potency of ketamine racemate. |
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| 言語 | en | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | intravenous anesthetics | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | ketamine | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | nicorandil | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | potassium channel | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | patch-clamp configuration | |||||||||||||||||||
| bibliographic_information |
en : The journal of medical investigation : JMI 巻 57, 号 3-4, p. 237-244, 発行日 2010-08 |
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| 収録物ID | ||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 13431420 | |||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||
| 収録物識別子 | AA11166929 | |||||||||||||||||||
| 備考 | ||||||||||||||||||||
| 言語 | ja | |||||||||||||||||||
| 値 | The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html | |||||||||||||||||||
| item_1723180141928 | ||||||||||||||||||||
| 識別子 | 233661 | |||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||
