性分化と精子形成機構に対するプロテオミクスからのアプローチ

SRY （sex determining region on the Y chromosome） was a gene isolated as the testis determining factor on the Y chromosome in １９９０．The gene consisted of single exon and encodes a ２０４ amino acid protein. Since SRY protein has a HMG domain, which functions as DNA binding domain, it has been considered to be a transcription factor. Direct target of SRY, however, have not yet identified for １４ years.
In this study, we have performed a proteomics approach to analyze the function of SRY. Twodimensional gel electrophoresis after over expression of SRY showed considerable down regulation in many proteins. Among the proteins, mitochondrial ６０‐kDa heat shock protein （HSP６０） and probable protein disulfide isomerase （ER６０） remarkably decreased. Since they were reported to be associated with cell proliferation and differentiation, we investigated the effects of SRY on cell cycle profiles. It was shown that the over‐expression of SRY negatively regulated the cell growth with significant S or G２／M arrest of cell cycle.

キーワード

主題

SRY

キーワード

主題

sex determination

キーワード

主題

Y chromosome

キーワード

主題

proteomics

書誌情報

ja : 四国医学雑誌

巻 60, 号 5-6, p. 168-171, 発行日 2004-12-20

収録物ID

収録物識別子タイプ

ISSN

収録物識別子

00373699

収録物ID

収録物識別子タイプ

NCID

収録物識別子

AN00102041

出版者

徳島医学会

備考

値

四国医学雑誌 : http://www.tokushima-u.ac.jp/med/research/shikoku_acta_medica.html

EID

識別子

134576

言語

jpn

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