| Item type |
文献 / Documents(1) |
| 公開日 |
2017-06-05 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1038/srep31481 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Efficient Genome Editing in Apple Using a CRISPR/Cas9 system |
|
言語 |
en |
| 著者 |
ニシタニ, チカコ
ヒライ, ナルミ
コモリ, サダオ
ワダ, マサト
オカダ, カズマ
刑部, 敬史
ヤマモト, トシヤ
刑部, 祐里子
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system. Four guide RNAs (gRNAs) were designed and stably transformed with Cas9 separately in apple. Clear and partial albino phenotypes were observed in 31.8% of regenerated plantlets for one gRNA, and bi-allelic mutations in apple PDS were confirmed by DNA sequencing. In addition, an 18-bp gRNA also induced a targeted mutation. These CRIPSR/Cas9 induced-mutations in the apple genome suggest activation of the NHEJ pathway, but with some involvement also of the HR pathway. Our results demonstrate that genome editing can be practically applied to modify the apple genome. |
|
言語 |
en |
| 書誌情報 |
en : Scientific Reports
巻 6,
p. 31481,
発行日 2016-08-17
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20452322 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
Copyright ©2016 Authors.This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| EID |
|
|
識別子 |
318870 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
LID201706014002.pdf (1.59 MB)
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