| Item type |
文献 / Documents(1) |
| 公開日 |
2017-11-06 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_1843 |
|
資源タイプ |
other |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
大腸上皮細胞に発現するNADPH oxidase1（Nox1）の分子特性 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
ダイチョウ ジョウヒ サイボウ ニ ハツゲン スル NADPH oxidase1 Nox1 ノ ブンシ トクセイ |
|
言語 |
ja-Kana |
| タイトル別表記 |
|
|
その他のタイトル |
Molecular characterization of NADPH oxidase1 expressed on large intestinal epithelial cells |
|
言語 |
en |
| 著者 |
桑野, 由紀
河原, 司
児玉, 七重
仲井, 沙織
岸, 恭一
六反, 一仁
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
NADPH oxidase 1 (Nox1) is an isozyme of gp91-phox predominantly expressed in the human colon. In this study, we have established primary cultures of guinea pig large intestinal epithelial cells (LIEC). A great majority of the cultured cells (>90%) was surface mucous cells containing periodic acid-Schiff reaction-positive granules. Vimentin-positive fibroblasts were <1%, and macrophages were not contaminated. LIEC spontaneously produced superoxide anion (O2 －) at about 160 nmol/mg protein/h. O2 －-dependent formation of blue formazan particles from nitroblue tetrazolium was observed only on surface of mucous-producing cells, and these cells expressed Nox1 protein at plasma membrane and in the cytoplasm. They expressed p67-phox, p22-phox, and rac1, but not gp91-phox, p47-phox, p40-phox, and rac 2. Immunohistochemistry showed that Nox1, p 67-phox, and p 22-phox were predominantly expressed in surface mucous cells of human and guinea pig colonic mucosa. Human colon cancer cell lines (Caco2, T84, and HT29 cells) expressed Nox1, p22-phox, and rac1, but not the other NADPH components. These cells secreted O2 － at <5 nmol/mg protein/h. Caco2 cells possessed Toll-like receptor 5, and flagellin (FliC) from Salmonella enteritidis phosphorylated transforming growth factor-β-activated kinase 1 (TAK1) and TAK1-binding protein 1, and significantly up-regulated O2 － production. These results suggest that Nox1 expressed in colonic epithelial cells may regulate interactions between pathogenic bacteria and epithelial cells for host defense. |
|
言語 |
en |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Nox1 |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
large intestinal epithelial cells |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Toll-like receptor 5 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
flagellin |
| 書誌情報 |
ja : 四国医学雑誌
巻 59,
号 1-2,
p. 45-46,
発行日 2003-04-25
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00373699 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00102041 |
| 出版者 |
|
|
出版者 |
徳島医学会 |
|
言語 |
ja |
| 言語 |
|
|
言語 |
jpn |
sam_59_1-2_45.pdf (19.4 KB)
