| Item type |
文献 / Documents(1) |
| 公開日 |
2017-11-21 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Cloning, expression analysis, and tissue distribution of esp-1/testisin, a membrane-type serine protease from the rat |
|
言語 |
en |
| 著者 |
ナカムラ, ヤスオ
イノウエ, マサヒロ
奥村, 裕司
シオタ, マユミ
西川, 舞
荒瀬, 誠治
木戸, 博
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| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Esp-1/testisin, a serine protease abundantly expressed in human and mouse testis, is presumed to play an important role in the process of spermatogenesis and fertilization. In this study, we cloned an esp-1/testisin cDNA from rats, and analyzed its expression and tissue distribution. The isolated cDNA consisted of 1099 nucleotides with a single open reading frame encoding 328 amino acids and an expected molecular mass of 36.6 kDa. The deduced amino acid sequence of rat Esp-1/Testisin had 89% and 62% identity with its murine and human counterparts, respectively, and appeared to be a trypsin-type serine protease with a hydrophobic region at the C-terminus. By quantitative real-time polymerase chain reaction analysis, rat esp-1/testisin mRNA was predominantly expressed in testis, as in human and mouse. However, its immunohistochemical distribution was predominantly in the elongated spermatids at steps 12 to 19, and not in the primary spermatocytes and round spermatids. This different distribution profile suggests that Esp-1/Testisin plays a role in species-specific proteolytic events during spermatogenesis and fertilization. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
serine protease |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
testis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
elongated spermatids |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
spermatogenesis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
fertilization |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 50,
号 1-2,
p. 78-86,
発行日 2003
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| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| EID |
|
|
識別子 |
154898 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jmi_50_1-2_78.pdf (543 KB)
