| Item type |
文献 / Documents(1) |
| 公開日 |
2018-01-23 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.2152/jmi.52.65 |
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|
言語 |
ja |
|
|
関連名称 |
10.2152/jmi.52.65 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Inhibitory effects of asiatic acid and CPT-11 on growth of HT-29 cells |
|
言語 |
en |
| 著者 |
Bunpo, Piyawan
片岡, 佳子
有持, 秀喜
中山, 治之
桑原, 知巳
Vinitketkumnuen, Usanee
大西, 克成
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Asiatic acid is a pentacyclic triterpene contained inmedicinal plants. The cytotoxic effect of this compound and its augmentative effect on the anticancer drug irinotecan hydrochloride (CPT-11) were investigated in the human colon adenocarcinoma cell lineHT-29. Asiatic acid dose-dependently showed cytotoxicity in HT-29 cells. DNA fragmentation, annexin-positive apoptotic cells, andcaspase-3 activation were observed in a dose-dependent manner. Acaspase-3 inhibitor suppressed the DNA ladder formation in a concentration-dependent manner. Bcl-2 and Bcl-xL proteins were decreased by asiatic acid treatment. These results indicate that asiatic acid induced apoptosis inHT-29 cells viacaspase-3activation.Cytotoxic effectsof combined treatment with CPT-11 and asiatic acid on HT-29 cells were further examined. Simultaneous treatment or sequential exposure first to asiatic acid and then to CPT-11 showed an additive effect. Synergism was observed when cells were first exposed to CPT-11 and then to asiatic acid. These results suggest that asiatic acid can be used as an agent for increasing sensitivity of colon cancer cells to treatment with CPT-11 or as an agent for reducing adverse effects of CPT-11. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
asiatic acid |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
apoptosis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
CPT-11 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
combination |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cytotoxicity |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 52,
号 1-2,
p. 65-73,
発行日 2005-02
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| EID |
|
|
識別子 |
128058 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jmi_52_1-2_65.pdf (682 KB)
