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Single-amino acid mutation 66SR in Gag-matrix enhances viral single-cycle infectivity of R5-tropic HIV-1rmt
https://tokushima-u.repo.nii.ac.jp/records/2004471
https://tokushima-u.repo.nii.ac.jp/records/200447193bab433-0795-4cea-a1fa-b94ed6b39b33
| 名前 / ファイル | ライセンス | アクション |
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jmi_62_3-4_228.pdf (433 KB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2018-05-22 | |||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.2152/jmi.62.228 | |||||||||||||||||||||||
| 関連名称 | 10.2152/jmi.62.228 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Single-amino acid mutation 66SR in Gag-matrix enhances viral single-cycle infectivity of R5-tropic HIV-1rmt | |||||||||||||||||||||||
| 著者 |
土肥, 直哉
× 土肥, 直哉× サカイ, ヨウスケ
× 宮﨑, 恭行× 足立, 昭夫
WEKO
1594
× 野間口, 雅子
WEKO
246
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| 内容記述 | We recently constructed two rhesus macaque-tropic human immunodeficiency virus type 1 (HIV-1 rmt) clones with CXCR-4 or CCR5 tropism, but a CCR5-tropic HIV-1rmt clone grew more poorly than a CXCR4topic clone. It has been demonstrated that interaction between viral Gag-matrix (MA) and Env-gp41 cytoplasmic tail is important for virion-incorporation of Env. Concordantly, Gag-MA mutations (62QR and 66SR) that rescue defects in virion-incorporation of Env/viral replication were reported. In this study, we analyzed effects of these Gag-MA mutations on R5-tropic HIV-1rmt replication potentials. While introduction of 62QR into three HIV-1rmt clones tested reduced their multi-cycle replication ability in rhesus lymphocytes or abolish single-cycle infectivity for luciferase reporter cells, three R5-tropic HIV-1rmt clones carrying 66SR exhibited similar growth kinetics to those of their parental clones. One such clone, 66SR+5gtu, appeared to induce stronger cytopathic effects than parental clone 5gtu. We therefore investigated effects of 66SR mutation on viral replication in more detail. Single-cycle infectivity of 66SR+5gtu was enhanced relative to that of 5gtu, but 66SR+5gtu virion production was significantly decreased compared to the 5gtu level. Gag-MA 66SR mutation may be useful to improve growth potentials of the R5-tropic HIV-1rmt clones. | |||||||||||||||||||||||
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| 主題 | HIV-1rmt | |||||||||||||||||||||||
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| 主題 | Gag-MA | |||||||||||||||||||||||
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| 主題 | Envelope | |||||||||||||||||||||||
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| 主題 | R5-tropic | |||||||||||||||||||||||
| 書誌情報 |
en : The Journal of Medical Investigation 巻 62, 号 3-4, p. 228-232, 発行日 2015-08 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 13496867 | |||||||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 13431420 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA11166929 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA12022913 | |||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | Faculty of Medicine Tokushima University | |||||||||||||||||||||||
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| 識別子 | 308178 | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
