| Item type |
文献 / Documents(1) |
| 公開日 |
2018-05-31 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1507/endocrj.46.199 |
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関連名称 |
10.1507/endocrj.46.199 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Infrequent Detectable Somatic Mutations of the RET and Glial Cell Line-derived Neurotrophic Factor (GDNF) Genes in Human Pituitary Adenomas |
| タイトル別表記 |
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その他のタイトル |
MUTATIONS OF RET AND GDNF GENES IN PITUITARY ADENOMAS |
| 著者 |
吉本, 勝彦
(田中)小杉, 知里
モリタニ, マキ
シミズ, エイジ
山岡, 孝
ヤマダ, ショウゾウ
佐野, 壽昭
板倉, 光夫
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| 抄録 |
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内容記述 |
RET is a receptor tyrosine kinase expressed in neuroendocrine cells and tumors. RET is activated by a ligand complex comprising glial cell line-derived neurotrophic factor (GDNF) and GDNF receptor-α (GDNFR-α). Activating mutations of the RET proto-oncogene were found in multiple endocrine neoplasia (MEN) 2 and in sporadic medullary thyroid carcinoma and pheochromocytoma of neuroendocrine origin. Mutations of the RET proto-oncogene and the glial cell line-derived neurotrophic factor (GDNF) gene were examined in human pituitary tumors. No mutations of the RET proto-oncogene including the cysteine-rich region or codon 768 and 918 in the tyrosine kinase domain were detected in 172 human pituitary adenomas either by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) or by PCR-restriction fragment length polymorphism (RFLP). Further, somatic mutations of the GDNF gene in 33 human pituitary adenomas were not detected by PCR-SSCP. One polymorphism of the GDNF gene at codon 145 of TGC or TGT was observed in a prolactinoma. The RET proto-oncogene message was detected in a normal human pituitary gland or 4 of 4 human pituitary adenomas with reverse transcription (RT)-PCR, and in rodent pituitary tumor cell lines with Western blotting. The expression of GDNF gene was detected in 1 of 4 human somatotroph adenomas, 1 of 2 corticotroph adenomas, and 2 of 6 rodent pituitary tumor cell lines with RT-PCR. Based on these, it is concluded that somatic mutations of the RET proto-oncogene or the GDNF gene do not appear to play a major role in the pituitary tumorigenesis in examined tumors. |
| キーワード |
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|
主題 |
RET proto-oncogene |
| キーワード |
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|
主題 |
Glial cell line-derived neurotrophic factor (GDNF) gene |
| キーワード |
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|
主題 |
Mutations |
| キーワード |
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|
主題 |
Expression |
| キーワード |
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|
主題 |
Pituitary adenomas |
| 書誌情報 |
en : Endocrine Journal
巻 46,
号 1,
p. 199-207,
発行日 1999
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13484540 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
09188959 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12020190 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10901436 |
| 出版者 |
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|
出版者 |
The Japan Endocrine Society |
| EID |
|
|
識別子 |
72188 |
| 言語 |
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|
言語 |
eng |
endocrj_46_1_199.pdf (3.23 MB)
