| Item type |
文献 / Documents(1) |
| 公開日 |
2018-11-12 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1371/journal.pone.0148887 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1371/journal.pone.0148887 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
DNA Inversion Regulates Outer Membrane Vesicle Production in Bacteroides fragilis |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
OMV Production in Bacteroides fragilis |
|
言語 |
en |
| 著者 |
ナカヤマ イマオオジ, ハルユキ
| ja |
ナカヤマ イマオオジ, ハルユキ
|
| ja-Kana |
ナカヤマ イマオオジ, ハルユキ
|
| en |
Nakayama-Imaohji, Haruyuki
|
Search repository
弘田, 克彦
ヤマサキ, ヒサシ
ヨネダ, サオリ
ナリヤ, ヒロフミ
スズキ, モトオ
Secher, Thomas
三宅, 洋一郎
Oswald, Eric
ハヤシ, テツヤ
クワハラ, トモミ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Phase changes in Bacteroides fragilis, a member of the human colonic microbiota, mediate variations in a vast array of cell surface molecules, such as capsular polysaccharides and outer membrane proteins through DNA inversion. The results of the present study show that outer membrane vesicle (OMV) formation in this anaerobe is also controlled by DNA inversions at two distantly localized promoters, IVp-I and IVp-II that are associated with extracellular polysaccharide biosynthesis and the expression of outer membrane proteins. These promoter inversions are mediated by a single tyrosine recombinase encoded by BF2766 (orthologous to tsr19 in strain NCTC9343) in B. fragilis YCH46, which is located near IVp-I. A series of BF2766 mutants were constructed in which the two promoters were locked in different configurations (IVp-I/IVp-II = ON/ON, OFF/OFF, ON/OFF or OFF/ON). ON/ON B. fragilis mutants exhibited hypervesiculating, whereas the other mutants formed only a trace amount of OMVs. The hypervesiculating ON/ON mutants showed higher resistance to treatment with bile, LL-37, and human β-defensin 2. Incubation of wild-type cells with 5% bile increased the population of cells with the ON/ON genotype. These results indicate that B. fragilis regulates the formation of OMVs through DNA inversions at two distantly related promoter regions in response to membrane stress, although the mechanism underlying the interplay between the two regions controlled by the invertible promoters remains unknown. |
|
言語 |
en |
| 書誌情報 |
en : PLOS ONE
巻 11,
号 2,
p. e0148887,
発行日 2016-02-09
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
19326203 |
| 出版者 |
|
|
出版者 |
PLOS |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
Copyright: © 2016 Nakayama-Imaohji et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| EID |
|
|
識別子 |
315272 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
pone_11_2_e0148887.pdf (2.04 MB)
