| Item type |
文献 / Documents(1) |
| 公開日 |
2019-07-04 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.2152/jmi.66.93 |
|
|
言語 |
ja |
|
|
関連名称 |
10.2152/jmi.66.93 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Expression of NADPH oxidase and production of reactive oxygen species contribute to ureteric bud branching and nephrogenesis |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
NADPH oxidase and ROS in kidney growth |
|
言語 |
en |
| 著者 |
近藤, 秀治
マツウラ, サト
Ariunbold, Jamba
木下, ゆき子
漆原, 真樹
須賀, 健一
オザキ, ナツコ
永井, 隆
藤岡, 啓介
香美, 祥二
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Ureteric bud branching and nephrogenesis are performed through large-scale proliferation and apoptosis events during renal development. Reactive oxygen species (ROS), produced by NADPH oxidase, may contribute to cell behaviors, including proliferation and apoptosis. We investigated the role of NADPH oxidase expression and ROS production in developing kidneys. Immunohistochemistry revealed that NADPH oxidase components were expressed on epithelial cells in ureteric bud branches, as well as on immature glomerular cells and epithelial cells in nephrogenic zones. ROS production, detected by dihydroethidium assay, was strongly observed in ureteric bud branches and nephrogenic zones, corresponding with NADPH oxidase localization. Organ culture of E14 kidneys revealed that the inhibition of NADPH oxidase significantly reduced the number of ureteric bud branches and tips, consistent with reduced ROS production. This was associated with reduced expression of phosphorylated ERK1/2 and increased expression of cleaved caspase-3. Organ culture of E18 kidneys showed that the inhibition of NADPH oxidase reduced nephrogenic zone size, accompanied by reduced ROS production, fewer proliferating cell nuclear antigen-positive cells, lower p-ERK1/2 expression, and increased expression of cleaved caspase-3. These results demonstrate that ROS produced by NADPH oxidase might play an important role in ureteric bud branching and nephrogenesis by regulating proliferation and apoptosis. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
developing kidney |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
NADPH oxidase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
oxidative stress |
| 書誌情報 |
en : The Journal of Medical Investigation
巻 66,
号 1-2,
p. 93-98,
発行日 2019-02
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13496867 |
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12022913 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| 出版者 |
|
|
出版者 |
Tokushima University Faculty of Medicine |
|
言語 |
en |
| EID |
|
|
識別子 |
370175 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jmi_66_1-2_93.pdf (1.15 MB)
