| Item type |
文献 / Documents(1) |
| 公開日 |
2019-11-05 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
|
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|
関連識別子 |
https://doi.org/10.1038/labinvest.2017.11 |
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関連名称 |
10.1038/labinvest.2017.11 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
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|
タイトル |
Iron suppresses erythropoietin expression via oxidative stress-dependent hypoxia-inducible factor-2 alpha inactivation |
| タイトル別表記 |
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その他のタイトル |
Inhibitory action of iron on erythropoietin |
| タイトル別表記 |
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その他のタイトル |
Inhibitory action of iron on erythropoietin via oxidative stress-HIF-2α pathway |
| 著者 |
オオシマ, ケイスケ
池田, 康将
堀ノ内, 裕也
ワタナベ, ヒロアキ
濱野, 裕章
木平, 孝高
岸, 誠司
(井澤)石澤, 有紀
宮本, 理人
ヒラヤマ, タスク
永澤, 秀子
石澤, 啓介
土屋, 浩一郎
玉置, 俊晃
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| 抄録 |
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内容記述 |
Renal anemia is a major complication in chronic kidney disease (CKD). Iron supplementation, as well as erythropoiesis-stimulating agents, are widely used for treatment of renal anemia. However, excess iron causes oxidative stress via the Fenton reaction, and iron supplementation might damage remnant renal function including erythropoietin (EPO) production in CKD. EPO gene expression was suppressed in mice following direct iron treatment. Hypoxia-inducible factor-2 alpha (HIF-2α), a positive regulator of the EPO gene, was also diminished in the kidney of mice following iron treatment. Anemia-induced increase in EPO and HIF-2α expression was also inhibited by iron-treatment. In in vitro experiments using EPO-producing HepG2 cells, iron stimulation reduced the expression of the EPO gene, as well as HIF-2α. Moreover, iron treatment augmented oxidative stress, and iron-induced reduction of EPO and HIF-2α expression was restored by tempol, an anti-oxidant compound. HIF-2α interaction with the EPO promoter was inhibited by iron treatment, and was restored by tempol. These findings suggested that iron supplementation reduced EPO gene expression via an oxidative stress-HIF-2α-dependent signaling pathway. |
| 書誌情報 |
en : Laboratory Investigation
巻 97,
号 5,
p. 555-566,
発行日 2017-03-06
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
15300307 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00236837 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12189985 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00713961 |
| 出版者 |
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|
出版者 |
Springer Nature |
| 備考 |
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|
値 |
This is the Author's Accepted Manuscript of the following article: Iron suppresses erythropoietin expression via oxidative stress-dependent hypoxia-inducible factor-2 alpha inactivation, Keisuke Oshima, Yasumasa Ikeda, Yuya Horinouchi, Hiroaki Watanabe, Hirofumi Hamano, Yoshitaka Kihira, Seiji Kishi, Yuki Izawa-Ishizawa, Licht Miyamoto, Tasuku Hirayama, Hideko Nagasawa, Keisuke Ishizawa, Koichiro Tsuchiya & Toshiaki Tamaki, Laboratory Investigation 97, 555–566 (2017), which has been published in final form at https://doi.org/10.1038/labinvest.2017.11. |
| EID |
|
|
識別子 |
322994 |
| 言語 |
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|
言語 |
eng |
labinvest_97_5_555.pdf (2.56 MB)
