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Determination of binding affinity of poly‐γ‐glutamate to Shiga toxin
https://tokushima-u.repo.nii.ac.jp/records/2006904
https://tokushima-u.repo.nii.ac.jp/records/2006904a58dd256-9918-49aa-8672-114d94aff4e9
| 名前 / ファイル | ライセンス | アクション |
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jfbc_42_5_e12538.pdf (1.95 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||
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| 公開日 | 2019-11-08 | |||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||
| 関連識別子 | https://doi.org/10.1111/jfbc.12538 | |||||||||||||||||||
| 言語 | ja | |||||||||||||||||||
| 関連名称 | 10.1111/jfbc.12538 | |||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Determination of binding affinity of poly‐γ‐glutamate to Shiga toxin | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| タイトル別表記 | ||||||||||||||||||||
| その他のタイトル | Binding affinity of poly-γ-glutamate to Shiga toxin | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 著者 |
金丸, 芳
× 金丸, 芳× ゴトウ, ツキエ
× Badr, Hoida Ali
× 横井川, 久己男
WEKO
1242
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| 内容記述タイプ | Abstract | |||||||||||||||||||
| 内容記述 | We examined poly-γ-glutamate from natto, a Japanese fermented food, in the ability to adsorb Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2). The polymer was immobilized by direct coupling to EAH-SepharoseTM. The poly-γ-glutamate-Sepharose (about 10 mg of ligand/mL of gel) adsorbed Stx2, but not Stx1: its dissociation constant (Kd) against Stx2 was calculated to be 14.0 μM. To analyze the binding site of poly-γ-glutamate against Stx2, we similarly immobilized glutamate and glutarate. Glutamate- and glutarate-Sepharoses (each 7 μmol of ligand/mL of gel) similarly adsorbed Stx2, but not Stx1; Kd values against Stx2 were calculated to be 14.0 and 30.0, respectively, μM. The common structures of PGA-, glutamate-, and glutarate-Sepharoses were considered to be glutaryl groups. When we added the mixture of Stx2 and poly-γ-glutamate-Sepharose to Caco-2 cells (a human colon epithelial cell line), poly-γ-glutamate-Sepharose was found to reduce the cytotoxicity of Stx2. | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | Escherichia coli O157:H7 | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | poly-γ-glutamate | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | Shiga toxin | |||||||||||||||||||
| 書誌情報 |
en : Journal of Food Biochemistry 巻 42, 号 5, p. e12538, 発行日 2018-03-04 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 17454514 | |||||||||||||||||||
| 出版者 | ||||||||||||||||||||
| 出版者 | Wiley Periodicals | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 備考 | ||||||||||||||||||||
| 言語 | ja | |||||||||||||||||||
| 値 | This is the peer reviewed version of the following article: Kanemaru, K, Goto, T, Badr, HA, Yokoigawa, K. Determination of binding affinity of poly‐γ‐glutamate to Shiga toxin. J Food Biochem. 2018; 42:e12538, which has been published in final form at https://doi.org/10.1111/jfbc.12538. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | |||||||||||||||||||
| EID | ||||||||||||||||||||
| 識別子 | 336716 | |||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||
