| アイテムタイプ |
文献 / Documents(1) |
| 公開日 |
2020-11-19 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.3389/fpls.2018.00916 |
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関連名称 |
10.3389/fpls.2018.00916 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Efficient Multiplex Genome Editing Induces Precise, and Self-Ligated Type Mutations in Tomato Plants |
| タイトル別表記 |
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その他のタイトル |
Multiplex Genome Editing in Tomato |
| 著者 |
ハシモト, リョウスケ
ウエタ, リサ
アベ, チヒロ
刑部, 祐里子
刑部, 敬史
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| 抄録 |
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内容記述 |
Several expression systems for multiple guide RNA (gRNAs) have been developed in the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) system to induce multiple-gene modifications in plants. Here, we evaluated mutation efficiencies in the tomato genome using multiplex CRISPR/Cas9 vectors consisting of various Cas9 expression promoters with multiple gRNA expression combinations. In transgenic tomato calli induced with these vectors, mutation patterns varied depending on the promoters used to express Cas9. By using the tomato ELONGATION FACTOR-1α (SlEF1α) promoter to drive Cas9, occurrence of various types of mutations with high efficiency was detected in the tomato genome. Furthermore, sequence analysis showed that the majority of mutations using the multiplex system with the SlEF1α promoter corresponded to specific mutation pattern of deletions produced by self-ligation at two target sites of CRISPR/Cas9 with low mosaic mutations. These results suggest that optimizing the Cas9 expression promoter used in CRISPR/Cas9-mediated mutation improves multiplex genome editing, and could be used effectively to disrupt functional domains precisely in the tomato genome. |
| キーワード |
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主題 |
CRISPR/Cas9 |
| キーワード |
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主題 |
multiplex genome editing |
| キーワード |
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主題 |
SlEF1α promoter |
| キーワード |
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主題 |
tomato |
| キーワード |
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主題 |
tRNA |
| 書誌情報 |
en : Frontiers in Plant Science
巻 9,
p. 916,
発行日 2018-07-03
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1664462X |
| 出版者 |
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出版者 |
Frontiers Media SA |
| 権利情報 |
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権利情報 |
Copyright © 2018 Hashimoto, Ueta, Abe, Osakabe and Osakabe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| EID |
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識別子 |
340086 |
| 言語 |
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言語 |
eng |
fpls_9_916.pdf (2.89 MB)
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