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Kinetic analysis of substrate competition in enzymatic reactions with β-D-galactosidase by capillary electrophoresis / dynamic frontal analysis
https://tokushima-u.repo.nii.ac.jp/records/2007707
https://tokushima-u.repo.nii.ac.jp/records/20077074fdc03b3-9ec2-4cea-9927-37323226cc1f
| 名前 / ファイル | ライセンス | アクション |
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jpba_188_113390.pdf (978 KB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2020-06-15 | |||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.jpba.2020.113390 | |||||||||||||||||||||||
| 関連名称 | 10.1016/j.jpba.2020.113390 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Kinetic analysis of substrate competition in enzymatic reactions with β-D-galactosidase by capillary electrophoresis / dynamic frontal analysis | |||||||||||||||||||||||
| 著者 |
ミネ, マサノリ
× ミネ, マサノリ
× 水口, 仁志
WEKO
807
× 髙栁, 俊夫
WEKO
549
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| 抄録 | ||||||||||||||||||||||||
| 内容記述 | Competitive inhibition between two substrates with an enzyme is investigated by capillary electrophoresis/dynamic frontal analysis (CE/DFA). Enzymatic hydrolyses of o-nitrophenyl β-D-galactopyranoside and p-nitrophenyl β-D-galactopyranoside with β-D-galactosidase were examined as a model competitive reaction. A sample solution containing the two substrates was injected into a capillary filled with a separation buffer containing an enzyme. Enzymatic hydrolysis occurred during the electrophoresis, and the products of o-nitrophenol and p-nitrophenol were continuously formed and resolved from the sample zone. Two-steps plateau signal was detected with the two-substrate solutions based on the difference in the effective electrophoretic mobility of o-nitrophenol and p-nitrophenol. Michaelis-Menten constants and inhibition constants were determined with the plateau heights. Usefulness of CE/DFA on competitive inhibition analysis is demonstrated in this study. | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | Capillary electrophoresis | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | Dynamic frontal analysis | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | β-D-galactosidase | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | Substrate competition | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | Michaelis-Menten constant | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | Inhibition constant | |||||||||||||||||||||||
| 書誌情報 |
en : Journal of Pharmaceutical and Biomedical Analysis 巻 188, p. 113390, 発行日 2020-05-29 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 07317085 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA10644220 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA11533870 | |||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | Elsevier | |||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||
| 権利情報 | © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||||||||||||||||||||
| EID | ||||||||||||||||||||||||
| 識別子 | 366090 | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
