| Item type |
文献 / Documents(1) |
| 公開日 |
2020-12-24 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1538/expanim.18-0152 |
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|
言語 |
ja |
|
|
関連名称 |
10.1538/expanim.18-0152 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Analysis of amino acid profiles of blood over time and biomarkers associated with non-alcoholic steatohepatitis in STAM mice |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
AMINO ACID PROFILES IN MICE WITH NASH |
|
言語 |
en |
| 著者 |
イイダ, アヤカ
クラヌキ, サチ
ヤマモト, リョウコ
ウチダ, マサヤ
オオタ, マサノリ
(市村)清水, 真祐子
常山, 幸一
マサキ, タカユキ
セイケ, マサタカ
ナカムラ, ツヨシ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The changes in free amino acid (AA) levels in blood during the progression from non-alcoholic steatohepatitis (NASH) to hepatocellular carcinoma (HCC) are unclear. We investigated serum AA levels, along with biochemical and histological events, in a mouse model of NASH. We induced NASH in male C57BL/6J mice with a streptozotocin injection and high-fat diet after 4 weeks of age (STAM group). We chronologically (6, 8, 10, 12, and 16 weeks, n=4–12 mice/group) evaluated the progression from steatohepatitis to HCC by biochemical and histological analyses. The serum AA levels were determined using an AA analyzer. Serum aspartate aminotransferase and alanine aminotransferase levels were higher in the STAM group than in the normal group (non-NASH-induced mice). Histological analysis revealed that STAM mice had fatty liver, NASH, and fibrosis at 6, 8, and 10 weeks, respectively. Moreover, the mice exhibited fibrosis and HCC at 16 weeks. The serum branched-chain AA levels were higher in the STAM group than in the normal group, especially at 8 and 10 weeks. The Fischer ratio decreased at 16 weeks in the STAM group, with increasing aromatic AA levels. These results suggested that this model sequentially depicts the development of fatty liver, NASH, cirrhosis, HCC, and AA metabolism disorders within a short experimental period. Additionally, serum amyloid A was suggested to be a useful inflammation biomarker associated with NASH. We believe that the STAM model will be useful for studying AA metabolism and/or pharmacological effects in NASH. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
amino acid metabolism disorder |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
diabetes |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
hyperlipidemia |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
non-alcoholic fatty liver disease |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
non-alcoholic steatohepatitis |
| 書誌情報 |
en : Experimental Animals
巻 68,
号 4,
p. 417-428,
発行日 2019
|
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13411357 |
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
18817122 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11032321 |
| 出版者 |
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|
出版者 |
Japanese Association for Laboratory Animal Science |
|
言語 |
en |
| 権利情報 |
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|
言語 |
en |
|
権利情報 |
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/4.0/>. |
| EID |
|
|
識別子 |
374666 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
expanim_68_4_417.pdf (4.83 MB)
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