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Kinetic analysis of the transphosphorylation with creatine kinase by pressure-assisted capillary electrophoresis/dynamic frontal analysis
https://tokushima-u.repo.nii.ac.jp/records/2008422
https://tokushima-u.repo.nii.ac.jp/records/2008422c34b17c2-7e9f-4e12-a356-fa7b6c8a788f
| 名前 / ファイル | ライセンス | アクション |
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abc_413_5_1453.pdf (1020 KB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2021-02-01 | |||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1007/s00216-020-03110-9 | |||||||||||||||||||||||
| 関連名称 | 10.1007/s00216-020-03110-9 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Kinetic analysis of the transphosphorylation with creatine kinase by pressure-assisted capillary electrophoresis/dynamic frontal analysis | |||||||||||||||||||||||
| 著者 |
ミネ, マサノリ
× ミネ, マサノリ
× 水口, 仁志
WEKO
807
× 髙栁, 俊夫
WEKO
549
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| 抄録 | ||||||||||||||||||||||||
| 内容記述 | Kinetic reactions of the transphosphorylation with creatine kinase (CK) were individually investigated between creatine (Cr) and creatine phosphate (CrP) by pressure-assisted capillary electrophoresis/dynamic frontal analysis (pCE/DFA). The transphosphorylations are reversible between Cr and CrP, and reverse reactions inevitably accompany in general batch analyses. In pCE/DFA, the kinetic reaction proceeds in a separation capillary and the product is continuously resolved from the substrate zone. Therefore, the formation rate is kept constant at the substrate zone without the reverse reaction, and the product is detected as a plateau signal. This study demonstrates the direct and individual analyses of both the forward and the backward kinetic reactions with CK by pCE/DFA. A plateau signal was detected in the pCE/DFA with ADP or ATP as one of the products on either the forward or the backward reactions. The Michaelis-Menten constants of Km,ATP (from Cr to CrP) and Km,ADP (from CrP to Cr) were successfully determined through the plateau signal. Determined values of Km,ATP and Km,ADP by pCE/DFA were smaller than the ones obtained by the pre-capillary batch analyses. The results agree with the fact that the reverse reaction is excluded in the analysis of the kinetic reactions. The proposed pCE/DFA is useful on individual analyses of both forward and backward kinetic reactions without any interference from the reverse reaction. | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | Capillary electrophoresis | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | Dynamic frontal analysis | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | Creatine kinase | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | Transphosphorylation | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | Kinetic analysis | |||||||||||||||||||||||
| 書誌情報 |
en : Analytical and Bioanalytical Chemistry 巻 413, 号 5, p. 1453-1460, 発行日 2021-01-21 |
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| 収録物ID | ||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 16182642 | |||||||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 16182650 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA11610734 | |||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | Springer Nature | |||||||||||||||||||||||
| 備考 | ||||||||||||||||||||||||
| 値 | This is a post-peer-review, pre-copyedit version of an article published in Analytical and Bioanalytical Chemistry. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00216-020-03110-9. | |||||||||||||||||||||||
| EID | ||||||||||||||||||||||||
| 識別子 | 372562 | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
