| Item type |
文献 / Documents(1) |
| 公開日 |
2021-03-02 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1111/jdi.13085 |
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|
言語 |
ja |
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|
関連名称 |
10.1111/jdi.13085 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Secreted factors from cultured dental pulp stem cells promoted neurite outgrowth of dorsal root ganglion neurons and ameliorated neural functions in streptozotocin‐induced diabetic mice |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Effect of SHED-CM on DPN |
|
言語 |
en |
| 著者 |
ミウラ ユラ, エミリ
ツネカワ, シン
ナルセ, ケイコ
ナカムラ, ノブヒサ
モテギ, ミキオ
ナカイ シモダ, ヒロミ
アサノ, サエコ
カトウ, マコト
ヤマダ, ユウイチロウ
イズモト アキタ, タカコ
山本, 朗仁
ヒメノ, タツヒト
コンドウ, マサキ
カトウ, ヨシロウ
ナカムラ, ジロウ
カミヤ, ヒデキ
|
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Aims/Introduction: Transplantation of stem cells promotes axonal regeneration and angiogenesis in a paracrine manner. In the present study, we examined whether the secreted factors in conditioned medium of stem cells from human exfoliated deciduous teeth (SHED‐CM) had beneficial effects on diabetic polyneuropathy in mice.
Materials and Methods: Conditioned medium of stem cells from human exfoliated deciduous teeth was collected 48 h after culturing in serum‐free Dulbecco's modified Eagle's medium (DMEM), and separated into four fractions according to molecular weight. Dorsal root ganglion neurons from C57BL/6J mice were cultured with SHED‐CM or DMEM to evaluate the effect on neurite outgrowth. Streptozotocin‐induced diabetic mice were injected with 100 μL of SHED‐CM or DMEM into the unilateral hindlimb muscles twice a week over a period of 4 weeks. Peripheral nerve functions were evaluated by the plantar test, and motor and sensory nerve conduction velocities. Intraepidermal nerve fiber densities, capillary number‐to‐muscle fiber ratio, capillary blood flow and morphometry of sural nerves were also evaluated.
Results: Conditioned medium of stem cells from human exfoliated deciduous teeth significantly promoted neurite outgrowth of dorsal root ganglion neurons compared with DMEM. Among four fractions of SHED‐CM, the only fraction of <6 kDa promoted the neurite outgrowth of dorsal root ganglion neurons. In addition, SHED‐CM significantly prevented decline in sensory nerve conduction velocities compared with DMEM in diabetic mice. Although SHED‐CM did not improve intraepidermal nerve fiber densities or morphometry of sural nerves, SHED‐CM ameliorated the capillary number‐to‐muscle fiber ratio and capillary blood flow.
Conclusions: These results suggested that SHED‐CM might have a therapeutic effect on diabetic polyneuropathy through promoting neurite outgrowth, and the increase in capillaries might contribute to the improvement of neural function. |
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言語 |
en |
| キーワード |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Conditioned medium |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Diabetic polyneuropathy |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Stem cells from human exfoliated deciduous teeth |
| 書誌情報 |
en : Journal of Diabetes Investigation
巻 11,
号 1,
p. 28-38,
発行日 2019-05-29
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20401124 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12488319 |
| 出版者 |
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出版者 |
Asian Association for the Study of Diabetes |
|
言語 |
en |
| 出版者 |
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|
出版者 |
John Wiley & Sons |
|
言語 |
en |
| 権利情報 |
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|
言語 |
en |
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| EID |
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|
識別子 |
355839 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jdi_11_1_28.pdf (1.65 MB)
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