| Item type |
文献 / Documents(1) |
| 公開日 |
2021-03-02 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1111/jdi.13214 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1111/jdi.13214 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Characterization of the taste receptor‐related G‐protein, α‐gustducin, in pancreatic β‐cells |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Characterization of α-gustducin |
|
言語 |
en |
| 著者 |
ウダガワ, ハルヒデ
ヒラモト, マサキ
カワグチ, ミホ
上番増, 喬
オハラ イマイズミ, ミカ
ナンモ, タカオ
ニシムラ, ワタル
ヤスダ, カズキ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Aims/Introduction: Taste receptors, T1rs and T2rs, and the taste‐selective G‐protein, α‐gustducin, are expressed outside the taste‐sensing system, such as enteroendocrine L cells. Here, we examined whether α‐gustducin also affects nutrition sensing and insulin secretion by pancreatic β‐cells.
Materials and Methods: The expression of α‐gustducin and taste receptors was evaluated in β‐cell lines, and in rat and mouse islets either by quantitative polymerase chain reaction or fluorescence immunostaining. The effects of α‐gustducin knockdown on insulin secretion and on cyclic adenosine monophosphate and intracellular Ca2+ levels in rat INS‐1 cells were estimated. Sucralose (taste receptor agonist)‐induced insulin secretion was investigated in INS‐1 cells with α‐gustducin suppression and in islets from mouse disease models.
Results: The expression of Tas1r3 and α‐gustducin was confirmed in β‐cell lines and pancreatic islets. Basal levels of cyclic adenosine monophosphate, intracellular calcium and insulin secretion were significantly enhanced with α‐gustducin knockdown in INS‐1 cells. The expression of α‐gustducin was decreased in high‐fat diet‐fed mice and in diabetic db/db mice. Sucralose‐induced insulin secretion was not attenuated in INS‐1 cells with α‐gustducin knockdown or in mouse islets with decreased expression of α‐gustducin.
Conclusions: α‐Gustducin is involved in the regulation of cyclic adenosine monophosphate, intracellular calcium levels and insulin secretion in pancreatic β‐cells in a manner independent of taste receptor signaling. α‐Gustducin might play a novel role in β‐cell physiology and the development of type 2 diabetes. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
α-Gustducin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Insulin secretion |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Taste receptor |
| 書誌情報 |
en : Journal of Diabetes Investigation
巻 11,
号 4,
p. 814-822,
発行日 2020-01-20
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| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20401124 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12488319 |
| 出版者 |
|
|
出版者 |
Asian Association for the Study of Diabetes |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
John Wiley & Sons |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
| EID |
|
|
識別子 |
364049 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jdi_11_4_814.pdf (1.64 MB)
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