| Item type |
文献 / Documents(1) |
| 公開日 |
2021-07-26 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.7554/ELIFE.55896 |
|
|
言語 |
ja |
|
|
関連名称 |
10.7554/ELIFE.55896 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
The peroxisome counteracts oxidative stresses by suppressing catalase import via Pex14 phosphorylation |
|
言語 |
en |
| 著者 |
オクモト, カンジ
Shermely, Mahmoud El
ナツイ, マサナオ
小迫, 英尊
ナツヤマ, リュウイチ
マルタニ, トシヒロ
フジキ, ユキオ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Most of peroxisomal matrix proteins including a hydrogen peroxide (H2O2)-decomposing enzyme, catalase, are imported in a peroxisome-targeting signal type-1 (PTS1)-dependent manner. However, little is known about regulation of the membrane-bound protein import machinery. Here, we report that Pex14, a central component of the protein translocation complex in peroxisomal membrane, is phosphorylated in response to oxidative stresses such as H2O2 in mammalian cells. The H2O2-induced phosphorylation of Pex14 at Ser232 suppresses peroxisomal import of catalase in vivo and selectively impairs in vitro the interaction of catalase with the Pex14-Pex5 complex. A phosphomimetic mutant Pex14-S232D elevates the level of cytosolic catalase, but not canonical PTS1-proteins, conferring higher cell resistance to H2O2. We thus suggest that the H2O2-induced phosphorylation of Pex14 spatiotemporally regulates peroxisomal import of catalase, functioning in counteracting action against oxidative stress by the increase of cytosolic catalase. |
|
言語 |
en |
| 書誌情報 |
en : eLife
巻 9,
p. e55896,
発行日 2020-08-24
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2050084X |
| 出版者 |
|
|
出版者 |
eLife Sciences Publications |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
| EID |
|
|
識別子 |
370208 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
elife_9_e55896.pdf (2.11 MB)
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