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Erosion of human X chromosome inactivation causes major remodeling of the iPSC proteome
https://tokushima-u.repo.nii.ac.jp/records/2009414
https://tokushima-u.repo.nii.ac.jp/records/20094140ea703f8-5b31-4469-9c16-377bc3624dd3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
celrep_35_4_109032.pdf (4.66 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||
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| 公開日 | 2021-12-07 | |||||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.celrep.2021.109032 | |||||||||||||||||||||||||||
| 関連名称 | 10.1016/j.celrep.2021.109032 | |||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||
| タイトル | Erosion of human X chromosome inactivation causes major remodeling of the iPSC proteome | |||||||||||||||||||||||||||
| 著者 |
Brenes, Alejandro J.
× Brenes, Alejandro J.
× 𠮷川, 治孝
WEKO
1190
× Bensaddek, Dalila
× Mirauta, Bogdan
× Seaton, Daniel
× Hukelmann, Jens L.
× Jiang, Hao
× Stegle, Oliver
× Lamond, Angus I.
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| 抄録 | ||||||||||||||||||||||||||||
| 内容記述 | X chromosome inactivation (XCI) is a dosage compensation mechanism in female mammals whereby transcription from one X chromosome is repressed. Analysis of human induced pluripotent stem cells (iPSCs) derived from female donors identified that low levels of XIST RNA correlated strongly with erosion of XCI. Proteomic analysis, RNA sequencing (RNA-seq), and polysome profiling showed that XCI erosion resulted in amplified RNA and protein expression from X-linked genes, providing a proteomic characterization of skewed dosage compensation. Increased protein expression was also detected from autosomal genes without an mRNA increase, thus altering the protein-RNA correlation between the X chromosome and autosomes. XCI-eroded lines display an ∼13% increase in total cell protein content, with increased ribosomal proteins, ribosome biogenesis and translation factors, and polysome levels. We conclude that XCI erosion in iPSCs causes a remodeling of the proteome, affecting the expression of a much wider range of proteins and disease-linked loci than previously realized. | |||||||||||||||||||||||||||
| 書誌情報 |
en : Cell Reports 巻 35, 号 4, p. 109032, 発行日 2021-04-27 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||
| 収録物識別子 | 22111247 | |||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||
| 出版者 | Elsevier | |||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||
| 権利情報 | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | |||||||||||||||||||||||||||
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| 識別子 | 376409 | |||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||
