| Item type |
文献 / Documents(1) |
| 公開日 |
2021-12-22 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
関連識別子 |
https://doi.org/10.2147/IDR.S299177 |
|
|
関連名称 |
10.2147/IDR.S299177 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Antibacterial Effects of Disulfiram in Helicobacter pylori |
| 著者 |
コバタケ, トモミ
オギノ, ケイキ
サカエ, ヒロユキ
ゴトウ, カズヨシ
渡辺, 朱理
マツシタ, オサム
オカダ, ヒロユキ
ヨコタ, ケンジ
|
| 抄録 |
|
|
内容記述 |
Background: Helicobacter pylori infection poses a risk of the occurrence of gastrointestinal diseases, such as gastric cancer. Its incidence rate is significantly reduced by eradication, and thereby, eradication therapy is generally performed. Disulfiram is an oral prescription drug mainly used for the treatment of alcohol dependence. In recent years, reports have been made on its anticancer and antibacterial effects, and thus, it has recently become an interesting subject. This study aimed to examine the antibacterial activity of disulfiram, investigate the presence or absence of its antibacterial activity on H. pylori, and determine whether it could be a new bactericidal drug against drug-resistant H. pylori.
Materials and Methods: Drug-sensitive strains of H. pylori and amoxicillin-resistant, clarithromycin-resistant, and metronidazole-resistant strains were used, and a growth inhibition test of H. pylori using disulfiram was performed. Furthermore, the expression of urease, vacuolating cytotoxin A (VacA), and CagA, the virulence proteins of H. pylori, was quantitatively analyzed using the Western blotting method. In addition, for H. pylori used in this study, the 16SrDNA sequence, a ribosomal gene involved in protein production, was analyzed to examine the presence or absence of gene mutation.
Results: Disulfiram suppressed the growth of 7 out of 12 H. pylori strains at 1 μg/mL, and no correlation was observed between their susceptibility/resistance to current eradication antimicrobial drugs and disulfiram resistance. Disulfiram reduced the expression levels of urease, VacA, and CagA proteins. H. pylori, which showed resistance to disulfiram, tended to have fewer gene deletions/insertions in the 16S rDNA sequence; however, no specific mutation was detected.
Conclusion: Disulfiram has a bactericidal effect on H. pylori at low concentrations, suggesting that it can be used as a supplement for current H. pylori eradication drugs. |
| キーワード |
|
|
主題 |
disulfiram |
| キーワード |
|
|
主題 |
Helicobacter pylori |
| キーワード |
|
|
主題 |
urease |
| キーワード |
|
|
主題 |
vacuolating toxin |
| キーワード |
|
|
主題 |
CagA |
| 書誌情報 |
en : Infection and Drug Resistance
巻 14,
p. 1757-1764,
発行日 2021-05-12
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
11786973 |
| 出版者 |
|
|
出版者 |
Dove Medical Press |
| 備考 |
|
|
値 |
所属英表記誤記あり (誤)Koch Medical School→(正)Kochi Medical School |
| 権利情報 |
|
|
権利情報 |
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms. php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
| EID |
|
|
識別子 |
377841 |
| 言語 |
|
|
言語 |
eng |
idr_14_1757.pdf (435 KB)
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