| Item type |
文献 / Documents(1) |
| 公開日 |
2021-12-23 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/ijms22115426 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3390/ijms22115426 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Epigenetic Distribution of Recombinant Plant Chromosome Fragments in a Human–Arabidopsis Hybrid Cell Line |
|
言語 |
en |
| 著者 |
Liaw, YengMun
Liu, Yikun
Teo, CheeHow
Cápal, Petr
和田, 直樹
フクイ, キイチ
Doležel, Jaroslav
オオミド, ノブコ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Methylation systems have been conserved during the divergence of plants and animals, although they are regulated by different pathways and enzymes. However, studies on the interactions of the epigenomes among evolutionarily distant organisms are lacking. To address this, we studied the epigenetic modification and gene expression of plant chromosome fragments (~30 Mb) in a human–Arabidopsis hybrid cell line. The whole-genome bisulfite sequencing results demonstrated that recombinant Arabidopsis DNA could retain its plant CG methylation levels even without functional plant methyltransferases, indicating that plant DNA methylation states can be maintained even in a different genomic background. The differential methylation analysis showed that the Arabidopsis DNA was undermethylated in the centromeric region and repetitive elements. Several Arabidopsis genes were still expressed, whereas the expression patterns were not related to the gene function. We concluded that the plant DNA did not maintain the original plant epigenomic landscapes and was under the control of the human genome. This study showed how two diverging genomes can coexist and provided insights into epigenetic modifications and their impact on the regulation of gene expressions between plant and animal genomes. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Arabidopsis genome |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA methylation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
epigenome |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
human–plant hybrid cell line |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
gene expression |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
whole-genome bisulfite sequencing (WGBS) |
| 書誌情報 |
en : International Journal of Molecular Sciences
巻 22,
号 11,
p. 5426,
発行日 2021-05-21
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
14220067 |
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
376263 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
ijms_22_11_5426.pdf (1.81 MB)
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