| Item type |
文献 / Documents(1) |
| 公開日 |
2021-12-03 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.2152/jmi.68.347 |
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関連名称 |
10.2152/jmi.68.347 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Effective in vitro differentiation of adipose-derived stem cells into Schwann-like cells with folic acid supplementation |
| タイトル別表記 |
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その他のタイトル |
Schwann-like cells from ADSCs using folic acid |
| 著者 |
Chen, Shuhai
池本, 哲也
徳永, 卓哉
オキカワ, ショウヘイ
宮崎, 克己
トクダ, カズノリ
山田, 眞一郎
齋藤, 裕
居村, 暁
森根, 裕二
島田, 光生
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| 抄録 |
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内容記述 |
Peripheral nerve injury (PNI) after pelvic surgery is a common issue with a significant impact on patients. Autologous nerve grafting is the gold standard treatment for PNI, but this technique cannot be applied to fine nerve fibers in the pelvis. Schwann-like cell (SLC) differentiation is a novel therapeutic strategy for this clinical condition. However, the efficiency of SLC differentiation remains unsatisfactory. We modified an SLC differentiation protocol using adipose-derived stem cells (ADSCs) and folic acid. Morphology, gene expression and secretion of neurotrophic factors were examined to assess the differentiation quality and phenotypic characteristics. Our new modified protocol effectively induced a Schwann cell (SC) phenotype in ADSCs as assessed by morphology and expression of SC markers [S100 calcium-binding protein B (S100B), P < 0.01 ; p75 neurotrophic receptor (p75NTR), P < 0.05]. SLCs produced by the new protocol displayed a repair phenotype with decreased expression of ERBB2 and early growth response protein 2 (EGR2) / KROX20 (P < 0.01). Furthermore, our new protocol enhanced both mRNA expression and secretion of nerve growth factors by SLCs (P < 0.01). This protocol enhanced the SC characteristics and functions of ADSC-derived SLCs. This promising protocol requires further research and may contribute to SC-based nerve regeneration. |
| キーワード |
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主題 |
Adipose-derived stem cell |
| キーワード |
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|
主題 |
Schwann-like cells |
| キーワード |
|
|
主題 |
Folic acid |
| キーワード |
|
|
主題 |
Nerve growth factor |
| キーワード |
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|
主題 |
Regenerative medicine |
| 書誌情報 |
en : The Journal of Medical Investigation
巻 68,
号 3-4,
p. 347-353,
発行日 2021-08
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13496867 |
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| 出版者 |
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|
出版者 |
Tokushima University Faculty of Medicine |
| EID |
|
|
識別子 |
386671 |
| 言語 |
|
|
言語 |
eng |
jmi_68_3-4_347.pdf (582 KB)
