| Item type |
文献 / Documents(1) |
| 公開日 |
2022-07-11 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.3390/app12073566 |
|
|
関連名称 |
10.3390/app12073566 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Establishment of an Epicutaneously Sensitized Murine Model of Shellfish Allergy and Evaluation of Skin Condition by Raman Microscopy |
| 著者 |
(市村)清水, 真祐子
イシマル, ソウイチロウ
Wai, Christine (Yee-Yan)
南川, 丈夫
常松, 貴明
エンドウ, アイコ
コジマ, タクミ
松本, 穣
小林, 智子
スミダ, サトシ
柿本, 拓海
宮上, 侑子
小川, 博久
尾矢, 剛志
常山, 幸一
|
| 抄録 |
|
|
内容記述 |
Background: Shellfish allergy is one of the most common food allergies. Recent studies have shown that sensitization to allergens via the skin is involved in the development of food allergies. In this study, a mouse model of shrimp allergy was generated by epicutaneous sensitization and used to identify skin conditions associated with susceptibility to sensitization. Methods: Four-week-old female BALB/c mice were sensitized by repeated application of 0.1 mg of tropomyosin to tape-stripped skin on days 0, 7, and 15, followed by a challenge on days 28 and 35. Results: Epicutaneously sensitized mice exhibited higher serum levels of tropomyosin-specific IgE on day 15 than control mice. After the oral challenge, model mice had higher anaphylaxis scores and lower rectal temperature. After three tape-strip treatments for sensitization, the skin was analyzed by Raman microscopy. The sensitized mice exhibited lower relative intensities of Raman bands at 399, 915, and 1073 cm−1 than control mice, which could be helpful noninvasive markers in screening for potential sensitization via the skin. Conclusions: An epicutaneous sensitization shellfish allergy model was generated. This model will be useful in studies to elucidate the pathogenesis of skin sensitization. Raman microscopy may also be valuable for capturing subtle skin changes leading to sensitization. |
| キーワード |
|
|
主題 |
food allergy |
| キーワード |
|
|
主題 |
epicutaneous sensitization |
| キーワード |
|
|
主題 |
shellfish |
| キーワード |
|
|
主題 |
shrimp |
| キーワード |
|
|
主題 |
tropomyosin |
| キーワード |
|
|
主題 |
skin |
| キーワード |
|
|
主題 |
Raman microscopy |
| キーワード |
|
|
主題 |
tape-strip |
| 書誌情報 |
en : Applied Sciences
巻 12,
号 7,
p. 3566,
発行日 2022-03-31
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20763417 |
| 出版者 |
|
|
出版者 |
MDPI |
| 権利情報 |
|
|
権利情報 |
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
384933 |
| 言語 |
|
|
言語 |
eng |
app_12_7_3566.pdf (1.25 MB)
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