| Item type |
文献 / Documents(1) |
| 公開日 |
2022-07-13 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s00125-021-05560-x |
|
|
言語 |
ja |
|
|
関連名称 |
10.1007/s00125-021-05560-x |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Characterisation of Ppy-lineage cells clarifies the functional heterogeneity of pancreatic beta cells in mice |
|
言語 |
en |
| 著者 |
フカイシ, タカヒロ
ナカガワ, ユウコ
フクナカ, アヤコ
サトウ, タカシ
ハラ, アケミ
ナカオ, ケイコ
サイトウ, ミチコ
コウノ, ケンジ
ミヤツカ, タケシ
田蒔, 基行
松久, 宗英
マツオカ, タカアキ
ヤマダ, テツヤ
ワタダ, ヒロタカ
フジタニ, ヨシオ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Aims/hypothesis
Pancreatic polypeptide (PP) cells, which secrete PP (encoded by the Ppy gene), are a minor population of pancreatic endocrine cells. Although it has been reported that the loss of beta cell identity might be associated with beta-to-PP cell-fate conversion, at present, little is known regarding the characteristics of Ppy-lineage cells.
Methods
We used Ppy-Cre driver mice and a PP-specific monoclonal antibody to investigate the association between Ppy-lineage cells and beta cells. The molecular profiles of endocrine cells were investigated by single-cell transcriptome analysis and the glucose responsiveness of beta cells was assessed by Ca2+ imaging. Diabetic conditions were experimentally induced in mice by either streptozotocin or diphtheria toxin.
Results
Ppy-lineage cells were found to contribute to the four major types of endocrine cells, including beta cells. Ppy-lineage beta cells are a minor subpopulation, accounting for 12–15% of total beta cells, and are mostly (81.2%) localised at the islet periphery. Unbiased single-cell analysis with a Ppy-lineage tracer demonstrated that beta cells are composed of seven clusters, which are categorised into two groups (i.e. Ppy-lineage and non-Ppy-lineage beta cells). These subpopulations of beta cells demonstrated distinct characteristics regarding their functionality and gene expression profiles. Ppy-lineage beta cells had a reduced glucose-stimulated Ca2+ signalling response and were increased in number in experimental diabetes models.
Conclusions/interpretation
Our results indicate that an unexpected degree of beta cell heterogeneity is defined by Ppy gene activation, providing valuable insight into the homeostatic regulation of pancreatic islets and future therapeutic strategies against diabetes. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Beta cell heterogeneity |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ca2+ imaging |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Diphtheria toxin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
GLUT2 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Pancreatic polypeptide |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ppy |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Single-cell RNA sequence |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Streptozotocin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
TSPAN8 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
UCN3 |
| 書誌情報 |
en : Diabetologia
巻 64,
号 12,
p. 2803-2816,
発行日 2021-09-09
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0012186X |
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
14320428 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00628104 |
| 出版者 |
|
|
出版者 |
Springer Nature |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
European Association for the Study of Diabetes |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
|
|
識別子 |
386005 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
diabetologia_64_12_2803.pdf (5.28 MB)
.png)
